摘要
为了探寻大白菜花青素含量快速测定的方法,本文利用pH差计法和色差仪分别测定了‘城阳青’(普通大白菜)、‘秋宝黄’(黄心白菜)和‘彩凤’3个不同叶色大白菜品种花青素含量以及色差指标,并对6个色泽参数与花青素含量进行了相关和回归分析。结果表明:6个色泽参数均能反映白菜叶片间叶色的细微变化,‘城阳青’和‘秋宝黄’不含花青素,紫色白菜‘彩凤’的花青素主要分布于由外向内的第1～4层叶片。色泽参数L,b,h和C分别与花青素含量呈极显著或显著负相关关系(相关系数分别为-0.85**,-0.58**,-0.49*,-0.58**)。以色泽参数值L、a、h与花青素含量建立了多元回归方程:y=90.24-1.07x1+5.65x2-142.71x3(x1为L值,x2为a值,x3为h值,y为花青素含量),该回归方程相关系数为0.9629。通过色差计依据建立的回归方程可快速测定紫色大白菜花青素的含量。
To find a fast method for determining anthocyanin content in Chinese cabbage [ Brassica campestris L. ssp. pekinensis （ Lour ） Olsson ], anthocyanin contents and color indices of 3 different colored Chinese cabbage cultivars were measured by the pH method and a colorimeter, respectively. Correlation and regression analysis between the color parameters and contents of anthocyanin was conducted. The results showed that the 6 color indices including L, a, b, C, h, and H could effectively reflect minor changes of leaf color; no anthocyanin was detected in cvs. ＇ Cheng yang qing＇ and ＇ Qiu bao huang＇. High contents of anthocyanin were detected in the 1 -gth leaf layer from outside to inside of the purple Chinese cabbage cv. ＇ Cai feng＇. Significant correlations were found between the color indices （ L, b, h, C } and contents of anthocyanin with correlation coefficients of - 0.85^ ＊ ＊ , - 0. 58 ^＊ ＊ , - 0.49 ^＊ , and - 0.58 ^＊ ＊ , respectively. Correlation coefficient of the regression equations was 0.9629. The regression equation obtained was as following： y = 90.24 - 1.07x1 ＋ 5.65x2 - 142.71x3 （ where xI was the L value; x2 was the a value; x3 was the h value; y was anthocyanin content）. Anthocyanin content in Chinese cabbage can be quickly determined by using a colorimeter and the regression equation.
出处
《青岛农业大学学报（自然科学版）》
2011年第3期201-204,共4页
Journal of Qingdao Agricultural University(Natural Science)
基金
国家科技支撑计划项目资助(2008BADB1B01)
关键词
紫色白菜
花青素
色差
相关性
回归分析
purple chinese cabbage
anthocyanin
color index
correlation
regression analysis
