摘要
本论文建立了超高效液相色谱-串联质谱法快速测定甘蔗中草甘膦的方法。以乙腈为萃取液,甘蔗中的草甘膦经加速溶剂萃取仪萃取,萃取液与9-芴基甲基三氯甲烷衍生化反应,应用超高效液相色谱-串联质谱仪定性定量法检测草甘膦,建立了定性定量检测甘蔗中草甘膦快速、准确、高灵敏度的方法。经方法学验证,该方法对草甘膦最低检出限LOD为0.05 mg/kg。在0.01～1μg/mL的线性范围中,相关系数r2>0.999,平均回收率为86.5%～100.6%。该方法灵敏度高、操作简单、定量准确、测定浓度范围广,是甘蔗样品中草甘膦含量检测的理想方法。
Super-high performance liquid chromatography-mass spectrum in series（UPLC-MS/MS） method was developed for the determination of glyphosate in sugar cane.Glyphosate in sugar cane was separated by ASE.The extracts were derivated with FMOC-Cl,and the glyphosate content was determined by UPLC-MS/MS.The limit of detection（LOD） for glyphosate was 0.05mg/kg.The linear range was 0.01-1 μg/mL with correlation coefficient above 0.999.The mean recovery was between 86.5 % and 100.6%.This method can be applied for the determination of glyphosate in sugar cane for its high sensitivity and high selectivity
出处
《现代食品科技》
EI
CAS
2011年第9期1143-1145,1156,共4页
Modern Food Science and Technology
