摘要
目的分析乙型肝炎病毒(HBV)DNA与HBV血清标志物之间的关系。方法应用酶联免疫吸附测定法(ELISA)和荧光定量PCR分别检测468例乙型肝炎患者血清标志物和HBV-DNA含量。结果 HBsAg、HBeAg、抗-HBc抗体和Pre-S1Ag阳性患者组的HBV-DNA阳性率(50.6%,χ2=56.28,P<0.001;97.7%,χ2=122.65,P<0.001;46.8%,χ2=8.46,P<0.01;62.4%,χ2=39.93,P<0.001)均显著高于相应的阴性患者组;抗-HBs抗体和抗-HBe抗体阳性患者组的HBV-DNA阳性率(2.1%,χ2=35.61,P<0.001;38.0%,χ2=6.34,P<0.05)均显著低于相应的阴性患者组。血清HBsAg含量和HBV-DNA拷贝数之间存在相关性(r2=0.028,P=0.016)。结论乙型肝炎患者血清HBV-DNA含量与HBV血清学标志物测定结果之间存在关联。
Objective To analyze the relationship between hepatitis B virus DNA （HBV-DNA） titer and HBV serological markers.Methods HBV-DNA and serological markers of 468 patients with hepatitis B virus were tested by enzyme linked immunosorbent assay （ELISA） and fluorescence quantitative PCR,respectively.Results The positive ratios of HBV-DNA in HBsAg,HBeAg,anti-HBc and Pre-S1Ag positive patients （50.6%,χ 2 =56.28,P0.001;97.7%,χ 2 =122.65,P0.001;46.8%,χ 2 =8.46,P0.01;62.4%,χ 2 =39.93,P0.001） were significantly higher than that in corresponding serological marker of negative ones,respectively.However,the positive ratios of HBV-DNA in anti-HBs and anti-HBe antibody positive patients （2.1%,χ 2 =35.61,P0.001;38.0%,χ 2 =6.34,P0.05） were significantly lower than that in corresponding serological marker of negative ones,respectively.There was a correlation between HBsAg and HBV-DNA copy numbers （r 2 =0.028,P=0.016）.Conclusion Some relationship exists between HBV-DNA titer and serological markers in patients with hepatitis B virus.
出处
《分子诊断与治疗杂志》
2011年第5期323-325,共3页
Journal of Molecular Diagnostics and Therapy
