摘要
采用N-羟基琥珀酰亚胺与双环己基碳酰二亚酰胺联用的方法将兽药头孢噻呋与牛血清白蛋白偶联,得到头孢噻呋人工牛血清抗原。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、电喷雾质谱(ESI MS)证实头孢噻呋人工牛血清抗原合成成功,计算得到头孢噻呋与牛血清蛋白的结合比约为8,并首次尝试将原子力显微镜(AFM)应用于头孢噻呋人工牛血清抗原的表征。
The ceftiofur artificial antigen was synthesized by conjugating ceftiofur to BSA with n-hydroxysuccinimide and N,N-dicyclohexyl carbodiimide.The synthesis product was characterized by the traditional sodium lauryl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） and electrospray ionization mass spectrometric（ESI MS） method.The result showed that the ceftiofur artificial bovine serum albumin antigen was succesfully prepared and the binary complex ratio of ceftiofur to BSA was about 8.Herein,atomic force microscope（AFM） was firstly used to characterize the ceftiofur artificial antigen.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第9期1013-1017,共5页
Journal of Instrumental Analysis
基金
国家科技支撑计划资助项目(2007BAD51B05)
关键词
头孢噻呋
人工抗原
原子力显微镜
ceftiofur
artificial antigen
atomic force microscope（AFM）
