摘要
为提高中国东北土著白腐真菌偏肿拟栓菌（Pseudotrametesgibbosa）对高分子量多环芳烃芘的共代谢降解效果，通过正交试验研究了共代谢基质、接种量、装液量及2,2＇-连氮-二（3-乙基苯并噻唑-6-磺酸）（ABTS）的最佳配比，同时采用浓度梯度法考察芘的初始浓度对偏肿拟栓菌共代谢降解芘的影响．结果表明，偏肿拟栓菌Pseudotrametesgibbosa共代谢降解芘的最佳培养基为：麸皮浓度为20WL，接种量为3片直径为10mm的菌片，装液量为50mL，不加ABTS，在该培养条件下，酶活可达53．41U／mL，对芘的降解率达到88．8％．初始浓度小于10mg／L的芘对菌体产酶有一定的促进作用，大于10mg／L时抑制漆酶的分泌，同时菌体对初始浓度小于90mg／L芘的降解率在22．24％-93．54％之间．
To enhance the degradation efficiency of pyrene co-metabolism by indigenous white-rot fungus Pseudotrametes gibbosa from northeast forest area in China, orthogonal experiments were designed to get the optimum conditions for pyrene co-metabolism, and the effects of different pyrene concentrations on the degradation efficiency of pyrene co-metabolism were also investigated. The optimum conditions were as following： wheat bran with the concentration of 20g/L, three agar plugs （10mm diameter） and 50mL culture in 150mL flask without ABTS. The maximum laccase （Lac） production and the degradation efficiency of pyrene reached 53.41U/mL and 88.8% respectively under the optimum conditions. Lac secretion was enhanced when pyrene concentration was lower than 10 mg/L, but it was inhibited at pyrene concentration above 10 mg/L. At the same time, the degradation efficiency of pyrene was ranged from 22.24%-93.54% if the initial concentration of pyrene was lower than 90 mg/L.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2011年第9期1516-1522,共7页
China Environmental Science
基金
全国优秀博士学位论文作者专项研究基金(200544)
黑龙江省杰出青年研究基金(JC200909)
关键词
共代谢
漆酶
偏肿拟栓菌
白腐真菌
芘
co-metabolism
laccase
Pseudotrametes gibbosa
white rot fungus： pyrene
