摘要
目的利用纤维素酶对苦参进行水解,以提高苦参有效成分的提取转移率、节省提取时间。方法在单因素试验基础上,以HPLC测得的苦参碱、氧化苦参碱的提取转移率为指标,采用星点试验设计,对加酶量(X1)、酶解pH(X2)和酶解时间(X3)3个因素(每个因素设5个水平)进行考察,优选苦参最佳酶解条件,并且以水提取方法作为对比,对酶解后的提取速度及次数进行考察。结果酶量为4 U/g、pH为5.6、温度为42℃时,纤维素酶对苦参的酶解效果最好,苦参碱、氧化苦参碱的提取转移率最高,在此条件下每次提取15 min,苦参碱、氧化苦参碱的提取转移率为87.89%,比水提取方法(不加酶、每次提取2 h)提高了7.03%,并节省了提取时间。结论纤维素酶的加入有利于苦参有效成分的溶出,节省提取时间,对工业生产有一定的意义。
Objective To carry out the hydrolysis for Kushen（Radix Sophorae Flavescentis） by using cellulose to increase the extraction transfer rate and save extraction time of active principles of Kushen.Methods On the foundation of single factor test,three factors,amount of cellulose addition（X1）,pH value of enzymolysis（X2） and time of enzymolysis（X3）,were observed（5 levels being set to each factor） by using central composite design taken the extraction transfer rate of matrine and oxymatrine obtained by HPLC as indexes.The best enzymolysis condition for Kushen was optimized and compared with traditional method.The extraction rate and times were investigated after enzymolysis.Results When the amount of cellulose was 4 U/g,pH was 5.6 and temperature was 42 ℃,the enzymatic effect of cellulose for Kushen was the best,and extraction transfer rate of matrine and oxymatrine was the highest.With this condition the extraction transfer rate of matrine and oxymatrine was 87.89% extracting for 15 minutes every time,which increased 7.03 percent and saved time compared with traditional method（not adding cellulose for 2 hour every time）.Conclusion Cellulose can destroy the structure of cell wall of Kushen material,improve the dissolution of active principles,and save the extraction time.It is significant to industry production.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2011年第8期552-555,576,I0001,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.81073059)
关键词
苦参
纤维素酶
星点设计
提取工艺
Kushen（Radix Sophorae Flavescentis）
cellulase
central composite design
extractive technique
