摘要
目的:运用现代生物技术探讨防风愈伤组织的培养条件。方法:以防风叶片为外植体,对防风的愈伤组织进行不同浓度生长素和细胞分裂素诱导。结果:在2,4-D1mg·L-1诱导形成的愈伤组织长势好于其他不同浓度,具有显著差异。生长素与细胞分裂素配合使用时,愈伤组织的诱导率明显高于单个生长素的处理,生长情况也有差异。结论:诱导防风最佳外源激素的浓度配比2,4-D1.0mg·L-1+6-BA0.5mg·L-1。
Objective：To investigate the culture conditions for callus induction in Saposhnikovia divaricata. Methods： With the blade of Saposhnikovia divaricata as explant, to induce the callus with different concentration of auxin and cytokinin. Results：The callus grew significantly better in conduction with 2,4-D 1 mg·L-1 than in other conditions. When auxin andcytokinins were used together, callus induction rate was significantly higher than single auxin. Conclusion： The optimal culture condition for callus induction is 2,4-D 1.0 mg· L - 1 ＋ 6-BA 0. 5 mg·L - 1
出处
《中国现代中药》
CAS
2011年第8期22-24,共3页
Modern Chinese Medicine
基金
吉林省中医药管理局项目--防风多倍体种质资源创新技术研究
关键词
防风
愈伤组织
诱导
激素
Saposhnikovia divaricata
Callus
Induction
Plant growth regulator
