摘要
背景:低氧诱导因子1α是介导低氧反应的核转录因子,其对肺纤维化的作用尚不明确。目的:观察低氧诱导因子1α在肺纤维化发病中的作用。方法:采用博来霉素(5mg/kg)一次性气管内灌注建立SD大鼠肺纤维化模型。造模后7,14,28d观察大鼠肺组织的病理学改变及肺组织中低氧诱导因子1α蛋白及mRNA的动态表达。结果与结论:博来霉素灌注后,大鼠肺组织炎症反应明显,并逐渐出现纤维化及胶原纤维沉积。免疫组织化学及RT-PCR结果显示,在大鼠肺纤维化发病过程中,低氧诱导因子1α蛋白及mRNA的表达趋势相同,即随造模时间的延长表达逐渐增多,并于造模后第14天达高峰。提示低氧诱导因子1α在大鼠肺纤维化发病过程中起重要作用,其过度表达可能参与了肺纤维化的发生与发展。
BACKGROUND：Hypoxia inducible factor 1α（HIF-1α） is a nuclear transcription factor to mediate hypoxia reaction. However, the effects of HIF-1α on lung fibrosis remain poorly understood. OBJECTIVE：To observe the effects of HIF-1α in the pathogenesis of pulmonary fibrosis. METHODS：Rat pulmonary fibrosis was induced by an intratracheal injection of bleomycin （5 mg/kg）. The pathogenetic procedure of pulmonary fibrosis and dynamic expression of HIF-1α in lung tissues were observed through some methods including hematoxylin-eosin and Masson＇s trichrome of lung section, immunohistochemical staining of HIF-1α protein in the lung, RT-PCR of HIF-1α mRNA in the lung on the 7th,14th, and 28th days. RESULTS AND CONCLUSION：In the pathogenetic procedure of pulmonary fibrosis in rats, compared with the normal group, the protein and mRNA expression of HIF-1α in the model group increased on the 7th day （10.33±0.31, 21.10±1.6, P 0.05） and the 14th day （17.65±1.82, 64.93±4.88, P 0.05）, but decreased on the 28th day （13.48±1.45, 47.85±3.40, P 0.05）. HIF-1α protein and mRNA was dynamically expressed in the lung tissue of rats with experimental pulmonary fibrosis, which may contribute to the pathogenesis of pulmonary fibrosis.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第28期5213-5216,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
湖南省自然科学基金资助项目(08JJ5003),课题名称:肺纤维化基因时空表达谱及黄芪甲甙干预的研究
湖南省财政厅/教育厅基金资助重点项目(10A107),课题名称:ADAMTS-1在肺纤维化中的作用及其机制研究
湖南省财政厅/教育厅基金资助一般项目(10C1160),课题名称:白黎芦醇逆转肺纤维化作用及MAPK/ERK信号转导特征研究
衡阳市科技发展基金资助项目(2010ks33),课题名称:白藜芦醇对大鼠肺纤维化组织中HIF-1α和NF-κB的表达影响~~
