摘要
目的 观察人免疫缺陷病毒（HIV）感染者辅助性T细胞（Thelpcell，Th）17和调节性T细胞（regula—toryTcell，Treg）的数量、平衡状态变化及其意义。方法选取确诊的106例HIV感染者和30例健康体检者（健康对照组），采集静脉血，以流式细胞术检测CD4＋T细胞及Th17细胞与Treg细胞百分比，RT-PCR检测HWRNA病毒载量。结果HIV感染者CD4＋T细胞为13～702个／μL，分为4组，即A组：≤200个／μL，19例；B组：201-350个／μL，32例；C组：351～500个／止，26例；D组：500个／uL，29例；上述各组均明显低于健康对照组（798-1130个／μL）（P〈（）．05）。A、B、C、D组Th17细胞在CD4＋T细胞中的百分比分别为（0．73±0．21）％、（1．52±0．33）％、（2．92±0．46）％、（3．74±0．58）％，均低于健康对照组的（4．90±1．43）％（P〈0．05）；A、B、C、D组及健康对照组Treg细胞在CD4＋T细胞中的百分比分别为（9．78±2．15）％、（7．23±1．72）％、（5．69±1．67）％、（4．62±0．97）％、（4．43±0．97）％，除D组与健康对照组之差异无统计学意义（P〉0．05），其余组均高于健康对照组（P〈（0.05）。HIV感染者病毒载量水平为（0～5．36）×10^7拷贝／mL，平均（5．36±1．21）x106拷贝／mL；A、B、C、D组的病毒载量分别为（6．24±1．36）×10^7，拷贝／mL、（4．36±1．02）×10^6拷贝／mL、（5．78±1．67）×10^4拷贝／mL、（3．54±1．25）×10^3拷贝／mL，各组间比较，差异有统计学意义（P〈0．05），且与CD4＋T细胞计数呈负相关。结论HIV感染导致The7和CD4十T细胞下降、Th17和Treg细胞矢衡，与HIVRNA载量负相关；与疾病进展密切相关。
Objective To evaluate the significance of change in T help cell 17（Th17） and regulatory T cell （Treg） in HIV-infected patients. Methods 106 HIV-infected patients and 31） healthy volunteers were selected. Flow cytometry was used to detect the CD4 ＋ T , Th17 and Treg cells in peripheral blood. HIV RNA levels in serum were tested by RT-PCR. Results CD4 ＋ T cells were 13 - 702/uL, 19 patients were ≤200/uL （group A）, 32 were 201 - 350/uL（ group B）, 26 were 351 - 500/uL（group C）, 29 were 〉500/uL（group D） ; All were significantly lower than （798- 1 130/uL） in healthy control group（P〈0. 05）. The percentage of Th17 cells in CD4 ＋ T cells were （0. 73±0. 21）%,（1.52 ±0. 33）%, （2. 92±0. 46）% , and （3. 74±0. 58）% in group A, B ,C and D, respectively, which were all lower than （4. 90 ± 1.43）% of control group（P〈0. 05） The percentage of Treg cells in CD4 ＋ T calls were （9.78 ± 2. 15）%, （7. 23 ± 1. 72）%, （5.69±1.67）% , and （4. 62± 0. 97）% in group A, B, C and D, respectively, group A, B and C were higher than （4. 43 ±0. 97）% of control group（P〈0.05）, 6ut group D had no significant difference compared wkh control group（P〉0.05）. HIV RNA levels were （0 - 5.36） × 10^7copies/mL in HIV-infected patients, the average levels of HIV RNA were （5.36± 1.21） × 10^6copies/mL HIV RNA levels in group A,B,C and D were（6 24± 1.36） × 10^7copies/mL, （4. 36±1.02） × 10^6 copies/mL, （5. 78±1.67） × 10^4 cop-ies/mL,and （3. 54 ± 1.25） × 10^3 copies/mL , respectively, there was significant difference between each group（P〈0. 05）, HIV RNA levels were negatively correlated with CD4 ＋ T cells count. Conclusion HIV infection can result in the decrease of Th17 and CD4 ＋ T cells, imbalance of Th17/Treg cells, and negative correlation with HIV RNA levels.
出处
《中国感染控制杂志》
CAS
2011年第4期252-255,共4页
Chinese Journal of Infection Control
基金
国家十一五重大科技专项(2008ZX10001-008)
