摘要
目的:调查广西妇女生殖道高危型人乳头瘤病毒(HPV)感染现状及与宫颈上皮内瘤变(CIN)的关系。方法:应用第二代杂交捕获(HC2)或HPV基因分型检测技术,对4874例妇女进行HPV检测,高危型HPV感染者进行宫颈活组织检查。结果:①两种方法检测后,HPV阳性率为27.00%(1316/4874),其中高危型HPV阳性率为23.78%(1159/4874),低危型HPV阳性率为3.22%(157/4874)。高危型HPV阳性者CIN57例,占4.92%(57/1159),CINⅡ～Ⅲ29例,占2.50%(29/1159)。②HC2检测结果:检测2260例,其中高危型HPV阳性率24.96%(564/2260)。高危型HPV阳性者CIN29例,占5.14%(29/564),CINⅡ～Ⅲ15例,占2.66%(15/564)。并且,病毒负荷量<100ng/L的CINⅠ、CINⅡ～Ⅲ与病毒负荷量>100ng/L比较,差异有高度统计学意义(χ2=21.098,P=0.000)。③HPV基因分型检测结果:检测2614例,HPV阳性率28.77%(752/2614),其中高危型HPV阳性率为22.76%(595/2614),低危型阳性率为6.01%(157/2614)。基因分型高危型HPV阳性者CIN28例,占4.71%(28/595),其中CINⅡ～Ⅲ14例,占2.35%(14/595)。低危型HPV阳性者CIN3例,占1.91%(3/157),无高度病变病例。基因分型人群中16、52和58型检出率最高,其百分率依次为27.56%,25.21%和21.85%。结论:广西妇女宫颈高危型HPV感染阳性率及HPV阳性者宫颈高度病变检出率均较高。HC2检测方法显示,HPV阳性,病毒负荷量高,宫颈高度病变检出率高。HPV基因分型16、52和58型为广西人群最常见的3种高危型。
Objective：To investigate the prevalence of high risk human papillomavirus （HPV） infection and its correlation with Cervical Intraepithelial Neoplasia （CIN） in Guangxi province. Methods：HPV in 4874 women was detected by hybrid capture Ⅱ （HC2） or HPV genotyping assay. High-risk HPV samples were detected by cervical biopsy.Results：①Detection by two methods： HPV positive rate was 27.00%（1316/4874）. Positive rate of high-risk HPV infection was 23.78% （1159/4874）, low-risk HPV was 3.22%（157/4874）. In high risk HPV infection, 57 was CIN, account for 4.92% （57/1159）, in which CINⅡ～Ⅲ were 29 cases,account for 2.50%（29/1159）②Testing results by HC2： Among 2260 patients, the positive incidence of high-risk HPV infection was 24.96% （564/2260）, in which 29 patients were detected as CIN, account for 5.14%（29/564）, and 15 cases were detected as CINⅡ～Ⅲ, account for 2.66% （15/564）. There was statistical difference about viral loading dose between CINⅠ～Ⅲ with less than 100 ng/L and more than 100 ng/L（χ2=21.098,P=0.000）. ③ Results of HPV genotyping assay： Among 2614 patients, HPV positive rate was 28.77%（752/2614）, while the positive rate was 22.76%（595/2614）in high-risk HPV infection and 6.01%（157/2614）in low-risk HPV infection. 28 patients were CIN in high risk HPV infection by genotype, account for 4.71%（28/595）. 14 cases were detected as CINⅡ～Ⅲ, with a rate of 2.35%（14/595）. 3 cases were detected as CIN in low-risk HPV infection, account for 1.91%（3/157）. There was no high-grade lesion. The positive rate of HPV detection in genotyping assay group was 27.56% in type 16, 25.21% in type 52, and 21.85% in type 58, respectively. Conclusions：The positive rate of cervical high-risk HPV infection and detection rate of cervical high-grade lesion in positive HPV infection are higher in Guangxi province. HC2 test indicates that the higher the HPV viral loading dose, the higher the detection rate of high-grade lesion. HPV genotypes 16, 52 and 58 are the commonest high-risk genotypes in this area.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2011年第8期596-599,共4页
Journal of Practical Obstetrics and Gynecology
基金
广西壮族自治区卫生厅重点科研课题项目(合同号:2010062)
关键词
人乳头瘤病毒
宫颈上皮内瘤变
流行病学调查
Human Papillomavirus
Cervical intraepithelial neoplasia
Epidemiological investigation
