摘要
目的建立一种HPLC法快速测定北冬虫夏草和几种常见食用菌中腺苷和虫草素的方法。方法 Welch Material XB-C18色谱柱(250mm×4.6mm,5μm),以水-甲醇(85:15)为流动相,体积流量1.0mL/min,检测波长258nm,柱温40℃。结果腺苷在0.0232～0.4640μg、虫草素在0.0300～0.6000μg与峰面积线性关系良好,平均加样回收率分别为98.97%、99.15%,RSD分别为1.41%、1.01%。结论腺苷普遍存在于各种食用菌子实体中,而虫草素是虫草属真菌子实体中的特有活性成分。本法简便快速,为全面评估北冬虫夏草中核苷类成分的质量,推进人工培育北冬虫夏草替代野生冬虫夏草提供检测方法。
Objective To develop a rapid method by using HPLC to quantitatively determine adenosine and cordycepin in Cordyceps militaris products and common edible fungi. Methods The working conditions： column was Welch Material XB-C18 column （250 mm×4.6 mm, 5 μm）, mobile phase was composed of water-methanol （85︰15） with flow rate of 1.0 mL/min and 258 nm detection wavelength, and temperature was 40 ℃. Results The linearity range of adenosine and cordycepin were 0.023 2-0.464 0 and 0.030-0.600 μg. The average recovery of adenosine and cordycepin were 98.97% and 99.15% with the RSD of 1.41% and 1.01%, respectively. Conclusion Adenosine appears widely in common edible fungi, and cordycepin is characteristic in fruitbodies of Cordyceps （Fr.） Link. The HPLC method is simple and fast., and could lay a method on the comprehensive evaluation of the nucleoside components in C. militaris, and promot the cultured C. militaris to replace the natural C. sinensis resources.
出处
《现代药物与临床》
CAS
2011年第4期313-315,共3页
Drugs & Clinic
基金
国家863计划重点项目(2007AA021502)
关键词
北冬虫夏草
冬虫夏草
腺苷
虫草素
高效液相色谱
Cordyceps militaris （L.） Link.
Cordyceps sinensis （Berk.） Sacc.
adenosine
cordycepin
HPLC
