摘要
目的探讨牛磺酸对硫代乙酰胺(TAA)诱导大鼠肝性脑病(HE)的保护作用。方法将24只健康雄性SPF级Wistar大鼠按体重随机分为3组,分别为空白对照组、HE模型组、牛磺酸+HE模型组,每组8只。牛磺酸+HE模型组给予牛磺酸(100 mg/kg)灌胃染毒,染毒容量为5 ml/kg,每天1次,连续染毒30 d;空白对照组、HE模型组给予等量生理盐水灌胃。30天后,HE模型组、牛磺酸+HE模型组腹腔注射TAA,首日剂量为300 mg/kg,随后剂量减半(150 mg/kg),染毒容量为5 ml/kg。空白对照组每天腹腔注射等量生理盐水。待HE大鼠出现无自主性活动或昏睡昏迷等症状时处死所有大鼠。检测血清3-硝基酪氨酸(3-NT)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量及超氧化物歧化酶(SOD)、谷丙转氨酶(ALT)的活力;制作肝组织病理切片观察大鼠肝组织病理变化。结果与HE模型组比较,空白对照组和牛磺酸+HE模型组大鼠肝脏脏器系数较低,血清中ALT活力和3-NT、MDA含量较低,GSH含量和SOD活力较高,差异均有统计学意义(P<0.05),而空白对照组和牛磺酸+HE模型组大鼠肝脏脏器系数、血清中3-NT、MDA、GSH含量及SOD、ALT活力间比较,差异均无统计学意义。结论牛磺酸可以通过降低氧化-硝基化应激改善蛋白质和脂质化损伤,从而对TAA致大鼠HE起到保护作用。
Objective To investigate the protective effect of taurine against hepatic encephalopathy （HE） induced by thioacetamide in rats. Methods Twenty-four Wistar rats were randomly divided into 3 groups by weight （8 in each group）： the blank control group,the HE model group,the taurine pretreated HE model group. The rats in the taurine pretreated HE model group were treated with taurine through gavage （5 ml/kg） once a day for 30 days with the dosage of 100 mg/kg, the rats in the blank control group and the HE model group were treated with the normal saline at the same volume. After 30 days,all the groups except the blank control group were treated with thioacetamide （5 ml/kg） by intraperitoneal injection once a day for 2 days with the dosage of 300 mg/kg at the first day and 150 mg/kg at the second day. At the same time the rats in the blank control group were treated with normal saline at the same dose. When HE rats appeared no independent activities or symptoms of lethargy and coma, they were sacrificed to collect blood for the detection of 3-nitrotyrosine （3-NT）, malondialdehyde （MDA）, superoxide dismutase （SOD） , reduced glutathione （GSH） and alanine aminotransferase （ALT）. Pathological examination was conducted in the liver tissues. Results Compared with the HE group, the liver viscera coefficients and the serum contents of ALT,MDA,3-NT decreased conspicuously,and the GSH,SOD greatly increased in the black control group and taurine pretreated HE model group （P〈0.05）,and there were no significant differences were seen in the above indices between the black control group and taurine pretreated HE model group. Conclusion In this study, taurine presented a protective effect against hepatic encephalopathy induced by thioacetamide in rats, in which decreasing oxidative and nitrosative stresses may be involved.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第8期686-689,F0003,共5页
Journal of Environment and Health
