摘要
目的探讨亲环素A(cyclophilin A,CyPA)基因BamHⅠ多态性与急性胰腺炎的关系。方法用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析101例急性胰腺炎患者和123名健康对照人群的CyPA基因型及等位基因频率分布状况,同时测定白细胞数、血糖、血清钙离子和尿素氮。结果两组均存在3种基因型:AA型、AG型和GG型;急性胰腺炎组AA型频率高于对照组,GG型频率低于对照组,基因型构成比有明显差异(χ2=20.206,P<0.05);对照组G等位基因频率高于急性胰腺炎组,两组间等位基因频率有明显差异(χ2=22.364,P<0.05)。结论 AA型可能是致急性胰腺炎的危险因素,G等位基因可能具有降低正常人群患急性胰腺炎的作用,CyPA基因多态性可能与急性胰腺炎有相关性。
Objective To investigate the relationship between acute pancreatitis and the single nucleotide polymorphisms（SNPs） of cyclophilin A gene.Methods 101 patients with acute pancreatitis and 123 normal controls were involved in the present study.The polymorphism of cyclophilin A gene was analyzed by PCR-RFLP and the serum level of glucose,calcium,blood urea nitrogen and white blood cell were measured by automatic biochemical analyzer or automatic blood cell analyzer.Results There were three kinds of genotype：AA,AG and GG;Significant difference was found in genotype and allele frequencies of cyclophilin A gene polymorphism between acute pancreatitis group and the control group.The frequency of AA genotype in acute pancreatitis group was higher than that of control group（χ2=20.206,P0.05）;The G allele frequency in acute pancreatitis group was lower than that of control group（χ2=22.364,P0.05）.Conclusion The G allele of cyclophilin A gene may be protective against the acute pancreatitis.It is confirmed that the polymorphism of cyclophilin A gene is associated with acute pancreatitis.
出处
《检验医学与临床》
CAS
2011年第15期1810-1811,1814,共3页
Laboratory Medicine and Clinic
关键词
亲环素A
基因多态性
急性胰腺炎
cyclophilin A
gene polymorphism
acute pancreatitis
