摘要
采用高效液相色谱法同时测定愈创木酚甘油醚、福尔可定和马来酸氯苯那敏的含量。色谱柱为C18（4.6mm×250 mm,5μm）,流动相为0.05 mol·L-1磷酸二氢钾溶液（每1000 mL加庚烷磺酸钠4.0 g、三乙胺1.0 mL,用磷酸调pH值至2.5）-乙腈-甲醇（50∶20∶30）,流速为1.0 mL.min-1,检测波长：愈创木酚甘油醚为224 nm,福尔可定和马来酸氯苯那敏为214 nm。结果表明,愈创木酚甘油醚在16.04~80.20μg.mL-1、福尔可定在7.96~39.80μg.mL-1、马来酸氯苯那敏在8.16~40.80μg.mL-1浓度范围内线性关系良好,R=0.9999,平均回收率（n=9）分别为99.2%、99.0%和99.5%。该方法简便、准确,三组分分离完全,可同时测定愈创木酚甘油醚、福尔可定和马来酸氯苯那敏的含量。
A method for determination of content of guaiphenesin,pholcodine and chlorphenamine maleate by HPLC at the same time was established.C18 Column（4.6 mm×250 mm,5 μm） was used with a mobile phase of 0.05 mol·L-1 monopotassium phosphate solution（containing 4.0 g·L-1 heptane sulfonic acid sodium and 1.0 mL·L-1 triethylamine,with pH value adjusted to 2.5 by phosphoric acid）-acetonitrile-methanol（50∶20∶30）and the flow rate was 1.0 mL·min-1.Guaiphenesin was detected at 224 nm,pholcodine and chlorphenamine maleate were detected at 214 nm.The results showed that guaiphenesin,pholcodine and chlorphenamine maleate presented a good linear relationship between amount and peak area（R=0.9999,n=5） within the range of 16.04～80.20 μg·mL-1,7.96～39.80 μg·mL-1 and 8.16～40.80 μg·mL-1,the average recovery（n=9） was 99.2%,99.0% and 99.5%,respectively.The method is simple and feasible.It is suitable for the determination of content of guaiphenesin,pholcodine and chlorphenamine maleate at the same time.
出处
《化学与生物工程》
CAS
2011年第7期89-91,共3页
Chemistry & Bioengineering
