摘要
为克隆人 B7.2 c DNA,构建 B7.2真核表达质粒 ,并在哺乳动物细胞中表达。分离正常人淋巴结淋巴细胞 ,经 LPS诱导后 ,以 RT- PCR,克隆 B7.2 c DNA;构建真核表达质粒 p BCMGSNeo- B7.2 ,转染哺乳细胞 ,进行表达。结果成功克隆了 B7.2c DNA,并经测序证实 :所构建的 B7.2抗原真核表达质粒可在哺乳动物细胞中高效表达。表明经 LPS诱导的人淋巴细胞可表达 B7.2 m RNA,所建立的 p BCMGSNeo-B7.2哺乳动物真核表达质粒 ,可供进一步研究 B7.2结构和功能。
Isolating lymphocytes from lymphonode of healthy human with LPS, cloning B7.2 cDNA by RT PCR;constructing eukaryotic expressing plasmid pBCMGSNeo B7.2 and then transfecting and expressing it in mammalian cells.The results showed that B7.2 cDNA was successfully cloned and verified by sequencing;constructed B7.2 antigen eukaryotic expressing plasmid was highly expressed in mammalian cells. These indicate human lymphocytes that induced with LPS can express B7.2 mRNA at high level;pBCMGSNeo B7.2 constructed eukaryotic expressing plasmid can be used in further study the struction and function of B7.2 molecule.
出处
《西安医科大学学报》
CSCD
1999年第4期441-444,共4页
Journal of Xi'an Medical University(Chinese)
基金
国家自然科学基金!No.394 70 2 93
