摘要
以大花萱草"御衣黄"花蕾为外植体建立其组织培养再生体系。研究结果表明,大花萱草"御衣黄"花蕾经75%乙醇30 s+0.1%升汞10 min处理后可获得较为理想的灭菌效果;诱导培养基MS+6-BA 5 mg/L+NAA 5mg/L是花蕾理想的脱分化培养基,愈伤组织诱导率达到75%;在降低了激素质量浓度的培养基MS+6-BA 1.5mg/L+NAA0.3 mg/L能促进愈伤组织增殖和分化,不定芽分化率45.7%;在生根培养基为1/2 MS+NAA 0.1mg/L中,组织培养苗生根率可达91.7%,且大花萱草"御衣黄"组织培养苗移栽1个月后成活率可达到100%。
An in vitro plant regeneration system of Hemerocallis middendorfii was established using buds as explants in the article.Results showed that the optimum sterilization method was 75% alcohol soaking 30 seconds and 0.1% HgCl2 treatment 10 minutes for explants.The optimum callus inducing medium was Murashige and Skoog（MS） ＋ NAA 0.5 mg/L ＋ 6-BA 3.0 mg/L with an induction rate of 75%.Callus was proliferated and adventitious buds were effectively generated on MS ＋ 6-BA 1.5 mg/L ＋ NAA 0.3 mg/L with a differentiation rate of 45.7%.The best rooting medium was 1/2 MS ＋ NAA 0.1 mg/L with a rooting rate 91.7%.The plantlet was transplanted with a transplanting survival rates up to 100% after one month.
出处
《江苏林业科技》
2011年第3期12-16,共5页
Journal of Jiangsu Forestry Science & Technology
基金
宁波市农业科研公关项目(2009C10019)
