摘要
目的运用小鼠植骨气囊模型,研究骨保护素(osteoprotegerin,OPG)减少破骨细胞骨破坏的吸收效应。方法在小鼠背部注入空气形成气囊,取同源小鼠的颅骨植入气囊内制作气囊模型。实验分成3组:空白组(气囊内注入生理盐水)、颗粒组(气囊注入聚乙烯颗粒和生理盐水)、OPG组(气囊内注入聚乙烯颗粒和OPG)。3周后取囊壁和植入颅骨组织进行HE染色检测炎症反应情况及炎症细胞渗出量;取组织匀浆液应用ELISA方法检测炎性细胞因子IL-1、TNF-α浓度;抗酒石酸酸性磷酸酶染色观察破骨细胞的分化成熟情况及应用扫描电镜检测骨片吸收情况。结果①HE染色检测炎症细胞渗出量:颗粒组[(3 812±628)个/视野]与OPG组[(3 665±297)个/视野]明显多于空白组[(1 820±598)个/视野,P<0.05]。②ELISA法检测炎性细胞因子IL-1、TNF-α浓度:颗粒组[IL-1、TNF-α浓度分别为(210.57±4.26)、(188.36±7.33)pg/ml]与OPG组[IL-1、TNF-α浓度分别为(198.59±6.90)、(179.28±2.11)pg/ml]明显多于空白组[IL-1、TNF-α浓度分别为(138.34±2.32)、(156.14±4.17)pg/ml,P<0.05]。③抗酒石酸酸性磷酸酶染色阳性区域面积与视野面积的百分比:颗粒组[(4.34±1.53)%]明显多于空白组[(0.89±0.12)%,P<0.05];OPG组[(0.96±0.55)%]与颗粒组相比,明显减少(P<0.05)。④应用扫描电镜检测骨片吸收陷窝面积与视野面积的百分比:颗粒组[(14.58±2.68)%]明显多于空白组[(3.09±0.62)%,P<0.05]。OPG组[(3.25±0.78)%]与颗粒组相比,有统计学差异(P<0.05)。讨论成功建立小鼠植骨气囊模型,并证实OPG对于聚乙烯颗粒所引起的炎症反应并未起到明显抑制作用,但OPG能抑制破骨细胞的分化成熟,有效减少聚乙烯磨损颗粒所致的骨吸收效应。
Objective To observe whether osteoprotegerin（OPG） is effective in reducing bone destruction and treating aseptic loosening in a mouse air pouch model.Methods Air was injected into the mouse back to form an air pouch,and the skull from another homologous mouse was implanted into the air pouch to obtain an air pouch model.Mice were divided into a blank group,a particle group,and an OPG group.Normal saline was injected into the air pouches of mice in the blank group.Polyethylene particles and physiological saline were injected into the air pouches of mice in the particle group.Polyethylene particles and OPG were injected into the air pouches of mice in the OPG group.Three weeks later,pouch walls and implanted skull tissues were collected,and the inflammatory reaction and the quantity of exuded inflammatory cells were detected through HE staining.The concentrations of inflammatory cytokines IL-1 and TNF-α in tissue homogenate were measured with ELISA.The differentiation and maturation of osteoclast were observed through tartrate-resistant acidic phosphatase staining.The resorption of bone slices was detected with scanning electron microscopy.Results The quantities of exuded inflammatory cells in the particle group[（3 812±628）/eyeshot] and the OPG group[（3 665±297）/eyeshot] were significantly higher than that in the blank group[（1 820±598）/eyeshot,P0.05].The IL-1 and TNF-α concentrations in the particle group[（210.57±4.26）,（188.36±7.33）pg/ml] and the OPG group [（198.59±6.90）,（179.28±2.11）pg/ml] were significantly higher than those in the blank group [（138.34±2.32）,（156.14±4.17）pg/ml,P0.05].The percentage of tartrate-resistant acidic phosphatase staining positive area to visual field area in the particle group（4.34±1.53）% was significantly higher than that in the blank group [（0.89±0.12）%,P0.05] and that in the OPG group [（0.96±0.55）%,P0.05].The percentage of bone slice resorption area to visual field area in the particle group（14.58±2.68）% was significantly higher than that in the blank group[（3.09±0.62）%,P0.05]and that in the OPG group [（3.25±0.78）%,P0.05].Conclusion Mouse air pouch model is established.OPG has no significant inhibitory effect on the inflammatory reaction induced by polyethylene wear particles,but can inhibit the differentiation and maturation of osteoclast and reduce the bone resorption induced by polyethylene wear particles.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2011年第14期1446-1450,共5页
Journal of Third Military Medical University
基金
国家自然科学基金(81071490/H0607)~~
