摘要
目的：评价冷血心肌麻痹液（ Ｃ Ｂ Ｃ）温度对心肌保护效果的影响． 方法：离体鼠心经 Ｃ Ｂ Ｃ不同温度（４，８，１２，１６ 和２０℃）停搏１２０ ｍ ｉｎ 及再灌注６０ ｍ ｉｎ， 测定心肌肌浆网（ Ｓ Ｒ） Ｃａ２＋ Ａ Ｔ Ｐａｓｅ 活性、钙摄取和 Ｓ Ｒ Ｃａ２＋ Ａ Ｔ Ｐａｓｅ ｍ Ｒ Ｎ Ａ 表达的改变． 结果：４，８，１２℃组 Ｓ Ｒ Ｃａ２＋ Ａ Ｔ Ｐａｓｅ 活性、钙摄取保护优于１６ 和２０℃组（ Ｐ＜ ０．０１），４℃～１６℃组 Ｓ Ｒ Ｃａ２＋ Ａ Ｔ Ｐａｓｅ ｍ Ｒ Ｎ Ａ 表达量为对照组１．１８～１．３２ 倍（ Ｐ＞ ０．０５），２０℃组表达量为对照组的 １．６３ 倍（ Ｐ＜ ０．０５）． 结论：温度影响 Ｃ Ｂ Ｃ心肌保护效果， Ｓ Ｒ Ｃａ２＋ Ａ Ｔ Ｐａｓｅ ｍ Ｒ Ｎ Ａ 表达上调可能与 Ｓ Ｒ泵功能受损的特异修复机制有关．
AIM: To evaluate the effect of temperature on myocardial protection during cold blood cardioplegic arrest. METHODS: Working hearts isolated from rats underwent 120 minutes of hypothermic blood cardioplegic arrest, during which myocardial temperatures were maintained at 4℃, 8℃, 12℃, 16℃ and 20℃ respectively. Alterations in sarcoplasmic reticulum (SR) Ca 2+ ATPase activity, Ca 2+ uptake and expression of SR Ca 2+ ATPase mRNA were assessed. RESULTS: SR Ca 2+ ATPase activity and Ca 2+ uptake in groups of 4℃, 8℃ and 12℃ were well maintained, which had better protective effects than those in groups of 16℃ and 20℃ ( P <0.01). Expression of SR Ca 2+ ATPase mRNA in groups of 4℃, 8℃, 12℃ and 16℃ did not increase significantly as compared with that of control group( P >0.05). However, upregulation of SR Ca 2+ ATPase mRNA in 20℃ group increased by 1.6 times ( P <0.05). CONCLUSION: Temperature has potential effects on myocardial protection during cold blood cardioplegic arrest. Protective efficacy attained by hypothermic arrest at temperatures from 4℃～12℃ may couple the provision of myocardial aerobic metabolism with the capacity to lower myocardial oxygen demands for arrested hearts. The upregualtion of SR Ca 2+ ATPase mRNA expression may suggest the specific repair mechanism of the impaired cardiac SR pump.
出处
《第四军医大学学报》
1999年第9期809-811,共3页
Journal of the Fourth Military Medical University
关键词
心麻痹液
温度
肌浆网
RNA
mRNA
ATP酶
cardioplegic solutions
temperature
sarcoplasmic reticulum
RNA
messenger
