摘要
目的研究人胃癌BGC-823肿瘤细胞系中是否存在悬浮生长的肿瘤球,检测前胃泌素(progastrin,Pro-GRP)在肿瘤球中的表达。方法取贴壁生长的BGC-823细胞,用有限稀释法和无血清培养法培养,并利用软琼脂克隆形成实验证实BGC-823中存在悬浮生长的具有干细胞特性的肿瘤球,流式细胞仪(FCM)检测该肿瘤球中ABCG2、CD44、CD166的表达。ELISA、细胞免疫荧光、FCM方法比较血清Pro-GRP分别在肿瘤球和贴壁细胞中的表达差异。结果有限稀释法和无血清培养法均在BGC-823细胞系中培养出了悬浮生长的肿瘤球;克隆形成能力显示:肿瘤球为(37.63±5.99),贴壁细胞为(10.75±3.37),二者间有统计学差异(P<0.01);FCM显示肿瘤球ABCG2的表达率(57.17±3.49)明显高于贴壁细胞(8.67±0.77)(P<0.01);血清Pro-GRP在肿瘤球中的表达(73.37±9.42)明显高于贴壁细胞(5.11±1.03)(P<0.01)。结论 人胃癌BGC-823细胞系中存在悬浮生长的肿瘤球,Pro-GRP的表达与肿瘤球有关。
Objective To explore whether there are suspension tumor spheres in human gastric BGC-823 cells,and investigate the expression of progastrin（Pro-GRP） in the tumor spheres.Methods Adherent cells were cultured by limit diluting and serum-free culture,then the existence of suspended cells that possessed the characteristic of stem cells was identified by colony formation capability assay.Flow cytometry was used to detect the expression of ABCG2 CD44 and CD166.The expression of Pro-GRP in the tumor spheres and the adherent cells was detected by ELISA,immunofluorescence and flow cytometry.Results The suspended tumor spheres were formed by limit diluting assay serum-free culture.The difference of colony formation was significant between suspended tumor spheres（37.63±5.99） and adherent cells（10.75±3.37）（P〈0.01）.The expression of ABCG2 in suspended tumor spheres was obviously higher than adherent cells（57.17±3.49 vs 8.67±0.77,P〈0.01）.The expression of Pro-GRP in suspended tumor spheres was markedly higher than adherent cells（73.37±9.42 vs 5.11±1.03,P〈0.01）.Conclusion Tumor spheres exist in human gastric BGC-823 cells,and Pro-GRP expression is related to tumor spheres.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2011年第13期1396-1399,共4页
Journal of Third Military Medical University
