期刊文献+

甘薯sporamin蛋白的功能及其在抗虫转基因植物中的应用 被引量:3

The Function of Sweet Potato's Special Tuber Storage Protein and Its Application in Engineering Plant Research
原文传递
导出
摘要 Sporamin蛋白是甘薯块根中一种主要的可溶性蛋白,具有块根特异表达、伤害诱导表达等生物学特性,并具有胰蛋白酶抑制剂活性。sporamin具有的胰蛋白酶抑制剂活性使其广泛应用于近年植物抗虫转基因的研究。该文就其序列结构特性、表达调控、生物学功能,以及在抗虫转基因领域的研究进展进行了综述。 Sporamin protein, a major soluble protein in sweet potato tuber, has root-specific, wound- induced expression pattern and Kunitz-type trypsin inhibitor activity. Potential protease inhibitor makes sporamin protein widely used in recent years' engineering plant research. This review is a summary of the current knowledge of sporamin protein on its structural features, expression regulation, biological activities as well as researches on en- gineering plant resistance against insects.
出处 《中国细胞生物学学报》 CAS CSCD 2011年第6期692-698,共7页 Chinese Journal of Cell Biology
基金 浙江省科技计划科研项目(No.2009C32026) 浙江省教育厅科研项目(No.20070173)资助项目~~
关键词 SPORAMIN 胰蛋白酶抑制剂 抗虫性 sporamin Kunitz-type trypsin inhibitor insect resistance
  • 相关文献

参考文献48

  • 1Walter AM Jr, Collins WW, Purcell AE. Sweet potato protein: A review. J Agric Food Chem 1984; 32: 695-9.
  • 2Maeshima M, Sasaki T, Asahi T. Characterization of major pro- teins in sweet potato tuberous roots. Photochem 1985; 24:1899- 902.
  • 3Jefferson R, Goldsbrough A, Bevan M. Transcriptional regulation ofa patatin-l gene in potato. Plant Mol Biol 1990; 4: 995-1006.
  • 4Shewry PR. Tuber strorage proteins. Ann Bot 2003; 91 : 755-69.
  • 5程龙军,郭得平,葛红娟.甘薯块根特异蛋白——Sporamin的研究进展[J].植物学通报,2001,18(6):672-677. 被引量:20
  • 6Murakami S, Hattori T, Nakamura K. Structural differences in full-length cDNAs for two classes of sporamin, the major soluble protein of sweet potato tuberous roots. Plant Mol Biol 1986; 7: 343-55.
  • 7Hattori T, Nakagawa T, Maeshima M, Nakamum K, Asahi T. Mo- lecular cloning and nucleotide sequence of cDNA for sporamin, the major soluble protein of sweet potato tuberous roots. Plant Mol Biol 1985; 5: 313-20.
  • 8Wang S J, Lin CT, Ho KC, Chen YM, Yeh KW. Nucleotide se- quence of sporamin gene in sweet potato. Plant Physiol 1995; 108: 829-30.
  • 9Hattori T, Nakamura k. Genes coding for the major tuberous root protein of sweet potato: Identification of putative regulatory se- quence in the 5' upstream region. Plant Mol Biol 1988; 11: 417- 26.
  • 10Yao PL, Hwang M J, Chen YM, Yeh KW. Site-directed mutagen- esis evidence for a negatively charged trypsin inhibitory loop in sweet potato sporamin. FEBS Lett 2001; 496: 134-8.

二级参考文献3

  • 1Yeh K W,Plant Mol Biol,1997年,33卷,565页
  • 2Chen J C,Taiwania,1997年,42卷,34页
  • 3Wang S J,Plant Physiol,1995年,108卷,829页

共引文献19

同被引文献66

引证文献3

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部