摘要
目的研究砷中毒后小鼠齿状回胶质原纤维酸性蛋白(GFAP)的变化。方法选取健康成年昆明小鼠80只,雌雄各半,分为对照组及慢性砷中毒高、中、低剂量组,每组20只,分别以蒸馏水、1/5 LD50、1/10 LD50、1/40LD50 As2O3连续灌胃3个月,根据其体质量变化随时调整用药剂量,采用Y-电迷宫检测各组小鼠学习记忆行为,采用免疫组织化学和蛋白印迹技术检测不同浓度砷中毒对小鼠齿状回部位GFAP表达的影响。结果 与正常对照组比较,高剂量砷中毒组学习、记忆Y-迷宫测试次数明显增多(P<0.05),小鼠齿状回GFAP阳性细胞明显增多(P<0.01),阳性反应产物平均光密度值增高(P<0.01),随砷中毒剂量的增加,小鼠齿状回GFAP蛋白含量随之增高(P<0.01)。结论 慢性砷中毒引起的学习记忆损伤,可能与齿状回神经胶质细胞反应性增生及GFAP表达增强有关。
Objective To investigate activation of glial fibrillary acidic protein（GFAP） in the dentate gyrus of adult mice after chronic arsenic poisoning. Methods 80 healthy adult Kunming mice, weighing 20-22g, were divided into four groups： the normal control group, the low-dose group, the medium-dose group and the high-dose group （ 10 males and 10 females in each group）. Mice in the four groups were respectively fed with distilled water, 1/5 LD50, 1/10 LD50 and 1/40 LD50 AS203 for 3 months, and the dosage was adjusted according to changes of weight. Then ability of learning and memory was tested by a Y-maze, and expression of the GFAP protein in the dentate gyrus by Western blot and immunohistochemistry. Results Ability of learning and memory in the high-dose group was significantly lower than that in the normal control group （P 〈0.05 ）. Immunohistochemical results showed that the number of GFAP-positive cells in the dentate gyms was significantly more increased in the dose groups compared with the normal control group（P 〈0.01 ）, and the average optical density was also increased （P 〈 0.01 ）. Western blot results showed the GFAP protein content increased with the dosage increasing （P 〈 0.01 ）. Conclusion Chronic arsenic poisoning might damage ability of learning and memory in mice, which may be related to proliferation of astroeytes and expression of GFAP in the dentate gyms.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第6期28-32,共5页
Journal of Shandong University:Health Sciences
基金
(2007)筑科计合同字第6-13号
(2009)筑科大合同字第4号
关键词
砷中毒
迷宫学习
胶质原纤维酸性蛋白
印迹法
蛋白质
齿状回
Arsenic poisoning
Maze learning
Glial fibfiUary acidic protein
Blotting, western
Dentate gyrus
