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mda7-/IL2-4和IL2-4delE5诱导急性髓系白血病细胞向单核细胞分化 被引量：3

mda-7/IL-24 and IL-24 delE5 induce myeloid leukemia cells to differentiate into monocytes
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摘要 目的:研究人黑素瘤分化相关基因-7(melanoma differentiation associated gene-7,mda-7;又称IL-24)及其剪接体IL-24 delE5与急性髓系白血病(acute myeloid leukemia,AML)细胞分化的关系。方法:十四烷酰佛波醇-乙酯(12-O-tetrade-canoylphorbol-13-acetate,TPA)处理人急性髓系白血病细胞系U937、HL-60,real-time PCR及Western blotting检测细胞中mda-7/IL-24和IL-24 delE5的表达,FACS检测细胞表面CD11b、CD14和CD115的表达。siRNA干扰U937、HL-60细胞中mda-7/IL-24和IL-24 delE5的表达后,再以TPA诱导细胞分化,FACS检测CD11b、CD14和CD115的表达。用前期构建的mda-7/IL-24和IL-24 delE5载体转染U937、HL-60及AML-M5患者的原代白血病细胞,观察异位过表达mda-7/IL-24和IL-24 delE5能否诱导白血病细胞向单核细胞分化。结果:TPA在诱导U937、HL-60细胞向单核细胞分化过程中显著促进mda-7/IL-24及其剪接体IL-24 delE5的表达,用siRNA干扰mda-7/IL-24及IL-24 delE5的表达后,TPA诱导U937、HL-60细胞向单核细胞分化的作用也被阻断。异位过表达mda-7/IL-24和IL-24 delE5可诱导U937、HL-60以及原代AML细胞向单核细胞分化:U937、HL-60细胞表面CD11b和CD14表达均显著升高,CD115表达仅在U937细胞有显著升高;3例AML-M5患者的原代AML细胞中CD11b、CD14及CD115表达均有不同程度升高,细胞呈现单核细胞的形态特征。结论:TPA可通过上调mda-7/IL-24及其剪接体IL-24 delE5的表达诱导白血病细胞向单核细胞分化。 Objective：To investigate the effects of melanoma differentiation associated gene-7（mda-7,also named IL-24） and IL-24 delE5,an mda-7/IL-24 splice variant,on differentiation of acute myeloid leukemia（AML） cells.Methods： The AML cell lines U937 and HL-60 were treated with 12-O-tetradecanoylphorbol-13-acetate（TPA）;mda-7/IL-24 and IL-24 delE5 expressions were detected by real-time PCR and Western blotting assays.CD11b,CD14 and CD115 expressions on cell surface were examined by FACS.U937 and HL-60 cells were induced with TPA after siRNA interfering mda-7/IL-24 and IL-24 delE5 expressions,and then CD11b,CD14 and CD115 expressions were examined by FACS.U937 and HL-60 cell lines and primary leukemia cells from AML-M5 patients were transfected with mda-7/IL-24 or IL-24 delE5 plasmids,which were established in our previous study,in order to know whether ectopic overexpressions of mda-7/IL-24 and IL-24 delE5 can induce the monocytic differentiation of leukemia cells.Results： The expressions of mda-7/IL-24 and its splice variant,IL-24 delE5,were significantly induced in U937 and HL-60 cells during TPA-mediated monocytic differentiation.Interfering mda-7/IL-24 and IL-24 delE5 expressions after siRNA treatment inhibited the monocytic differentiation ability of TPA on U937 and HL-60 cells.Ectopic overexpressions of mda-7/IL-24 and IL-24 delE5 induced differentiation of U937,HL-60,and primary AML leukemia cells into monocytes： CD11b,CD14 expressions on U937 and HL-60 cells and CD115 expression on U937 were significantly increased;CD11b,CD14 and CD115 expressions on primary leukemia cells from 3 AML patients were also increased with monocytic features.Conclusion： TPA can induce monocytic differentiation of leukemia cells by increasing mda-7/IL-24 and its splice variant IL-24 delE5 expressions.
作者 姚兴荣 马小彤 杨宾霞 段永娟 林永敏
机构地区 中国医学科学院北京协和医学院血液病医院暨血液学研究所实验血液学国家重点实验室
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2011年第3期244-250,共7页 Chinese Journal of Cancer Biotherapy
基金 国家自然科学基金资助项目(No.30872983 81070426) 天津市应用基础研究基金重点资助项目(No.11JCZDJC18000)~~
关键词 黑素瘤分化相关基因-7(mda-7/IL-24) IL-24delE5 十四烷酰佛波醇-乙酯 急性髓系白血病 单核细胞 分化 melanoma differentiation associated gene-7（mda-7/IL-24）； IL-24 delE5； 12-O-tetradecanoylphorbol-13-acetate（TPA）； acute myeloid leukemia； monocyte； differentiation
分类号 R733.7 [医药卫生—肿瘤] R730.2 [医药卫生—肿瘤]
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参考文献20

  • 1Jiang H, Lin JJ, Su ZZ, et al. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modula- ted during human melanoma differentiation,growth and progression [J]. Oneogene, 1995, 11 (12) : 2477-2486.
  • 2Yacoub A, Hamed HA, Allegood J, et al. PERK-dependent regu- lation of ceramide synthase 6 and thioredoxin play a key role in mda-7/IL-24-induced killing of primary human gliablastoma multiformecells [J]. CancerRes, 2010, 70(3): 1120-1129.
  • 3Gernmno IM, Emdad L, Qadeer ZA, et al. Embryonic stem cell (ESC)-mediated transgene delivery induces growth suppression, apoptosis and radiosensitization, and overcomes tenmzolomide re- sistance in malignant gliomas [J].Cancer Gene Ther, 2010, 17 (9) : 664-674.
  • 4Dash R, Richards JE, Su ZZ, et al. Mechanism by which Mcl-1 regulates cancer-specific apoptosis triggered by mda-7/IL-24, an IL-10-related cytokine [ J ]. Cancer Res, 2010, 70 ( 12 ) : 5034- 5045.
  • 5Park MA, Walker T, Martin AP, et al. mda-7/IL-24-induced cell killing in malignant renal carcinoma cells occurs by a ceramide/ CD95/PERK-dependcnt mechanism [ J ]. Mol Cancer Ther, 2009, 8(5) : 1280-1291.
  • 6Sauane M, Su ZZ, Gupta P, et al. Autocrine regulation of mda-7/ IL-24 mediates cancer-specific apoptosis [ J]. Proc Natl Acad Sci U S A, 2008, 105(28) : 9763-9768.
  • 7Zheng M, Bocangel D, Ramesh R, et al. Interleukin-24 overcomes temozolomide resistance and enhances cell death by down-regulation of O6-methylguanine-DNA methyhransferase in human melanoma cells [J].Mol Cancer Ther, 2008, 7 (12) : 3842- 3851.
  • 8Lebedeva IV, Sarkar D, Su ZZ, et al. Molecular target-based therapy of pancreatic cancer [ J ]. Cancer Res, 2006, 66 (4) : 2403 -2413.
  • 9Bhutia SK, Dash R, Das SK, et al. Mechanism of autophagy to apoptosis switch triggered in prostate cancer cells by antitumor cytokine melanoma differentiation-assoeiated gene 7/interleukin-24 [J]. CancerRes, 2010, 70(9): 3667-3676.
  • 10Lebedeva IV, Su ZZ, Vozhilla N, et al. Mechanism of in vitro pancreatic cancer cell growth inhibition by melanoma differentiation-associated gene-7/interleukin-24 and Cancer Res, 2008, 68 (18) : 7439-7447.

二级参考文献41

  • 1Xue, Xin-Bo,Chen, Kun,Wang, Cong-Jun,Zheng, Jian-Wei,Yu, Yuan,Peng, Zhi-Hai,Wu, Zai-De.Adenovirus vector expressing mda-7 selectively kills hepatocellular carcinoma cell line Hep3B[J].Hepatobiliary & Pancreatic Diseases International,2008,7(5):509-514. 被引量：6
  • 2Jiang H, Lin JJ, Su ZZ, et al. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation ,growth and progression [ J]. Oncogene. 1995, 11 (12) : 2477-2486.
  • 3Gupta P, Su ZZ, Lebedeva IV, et al. mda-7/lL-24: multifunctional cancer-specific apoptosis-inducing cytokine [ J]. Pharmacol Ther. 2006 , 111 ( 3 ) :596-628.
  • 4Su ZZ, Lebedeva IV, Sarkar D, et al. Melanoma differentiation associated gene-7, mda-7/1L-24, selectively induces growth sup- pression, apoptosis and radiosensitization in malignant gliomas in a p53-independent manner [ J ]. Oncogene. 2003,22 ( 8 ) : 1164-1180.
  • 5Sauane M, Gopalkrishnan RV, Sarkar D, et al. mda-7/lL-24: novel cancer growth suppressing and apoptosis inducing cytokine [J]. Cytokine Growth Factor Rev. 2003,14( 1 ) : 35-51.
  • 6Chada S, Sutton RB, Ekmekcioglu S, et al. mda-7/IL-24 is a unique eytokine-tumor suppressor in the IL-10 family [ J]. Int Immunopharmacol. , 2004 ,4 ( 5 ) :649-667.
  • 7Cunningham CC, Chada S, Merritt JA, et al. Clinical and local biological effects of an intratumoral injection of mda-7 ( IL24; INGN 241 ) in patients with advanced carcinoma: a phase I study [J]. Mol Ther, 2006,317(1) :207-218.
  • 8Tong AW, Nemunaitis J, Su D, et al. Intratumoral injection of INGN-241, a non-replicating adenovector expressing the melanoma-differentiation associated antigen-7 ( rada-7/lL-24 ) : biologic outcome in advanced cancer patients [J].Mol Ther, 2005,11 ( 1 ) : 160-172.
  • 9Lebedeva IV, Sarkar D, Su ZZ, et al. Molecular target-based therapy of pancreatic cancer [ J ]. Cancer Res, 2006,66 (4) : 2403 -2413.
  • 10Jiang H, Su ZZ, Lin J J, et al. The melanoma differentiation associated gene mda-7 suppresses cancer cell growth [ J]. Proc Natl Acad Sci USA ,1996,93(17) : 9160-9165.

共引文献5

同被引文献23

  • 1Siersema PD, van Hillegersberg R. Treatment of locally advanced esophageal cancer with surgery and chemoradiation [J]. CUIT Opin Gastroenterol, 2008, 24 ( 4 ) : 535 -540.
  • 2Ma G, Kawamura K, Li Q, et al. Cytotoxicity of adenoviruses expressing the wild-type p53 gene to esophageal carcinoma cells is linked with the CAR expression level and indirectly with the endogenous p53 status [J]. Cancer Gene Ther, 2009, 16 ( 11 ) : 832-840.
  • 3Shimada H, Matsubara H, Shiratori T, et al. Phase I I IT adenoviral p53 gene therapy for chemoradiation resistant advanced esophageal squamous cell carcinoma [J]. Cancer Sci, 2006, 97 (6) : 554-56l.
  • 4Dent P, Yacoub A, Hamed HA, et al. MDA-7/IL-24 as a cancer therapeutic: From bench to bedside [J] . Anticancer Drugs, 2010,21(8): 725-73l.
  • 5EMDAd L, Lebedeva IV, Su ZZ, et al. Historical perspective and recent insights into our understanding of the molecular and biochemical basis of the antitumor properties of MDA-7/IL-24 [J] . Cancer Bioi Ther, 2009, 8 (5) : 391-400.
  • 6WangM, TanZ, ZhangR, etal. Interleukin24 (MDA-7/MOB- 5) signals through two heterodimeric receptors, IL-22 RlIIL-20 R2 and IL-20RlIIL-20R2 [J]. J Bioi Chern, 2002, 277(9): 7341- 7347.
  • 7Pataer A, Hu W, Xiaolin L, et al. Adenoviral endoplasmic reticulum-targeted MDA-7/interleukin-24 vector enhances human cancer cell killing [J]. Mol Cancer Ther , 2008, 7(8): 2528-2535.
  • 8J Lebedeva IV, Su ZZ, Sarkar D, et al. Induction of reactive oxygen species renders mutant and wild-type K-ras pancreatic carcinoma cells susceptible to Ad. MDA-7-induced apoptosis [J]. Oncogene, 2005,24(4): 585-596.
  • 9Kreis S, Philippidou D, Margue C, et al. Recombinant interleukin-24 lacks apoptosis-inducing properties in melanoma cells [J] . PLoS ONE, 2007,2(12): -iaoo.
  • 10Su Z, EMDAd L, Sauane M, Lebedeva IV et al. Unique aspects of MDA 7 /1124 antitumor bystander activity: Establishing a role for secretion of MDA7/1124 protein by normal cells [J]. Oncogene, 2005, 24 (51 ) : 7552-7566.

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中国肿瘤生物治疗杂志
2011年 第3期

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