摘要
目的：建立HPLC法同时测定白术挥发油中苍术酮、β-桉叶醇、白术内酯Ⅰ、Ⅲ、β-榄香烯含量。方法：采用HypersilODS2（4.6 mm×250 mm,5μm）色谱柱;流动相乙腈（A）-水（B）60∶40,梯度洗脱[0~30 min,40%B→10%B;30~40 min,10%B→10%B];流速为1.0 mL.min-1,柱温30℃;检测波长：0~9 min时200 nm（白术内酯Ⅲ、β-桉叶醇）,9.1~20 min时220 nm（白术内酯Ⅰ）,20.1~45 min时200 nm（苍术酮、β-榄香烯）。结果：苍术酮浓度在0.10~1.00 mg.mL-1;β-桉叶醇、白术内酯Ⅰ、Ⅲ、β-榄香烯浓度分别在20.00~1000.00,3.00~150.00,4.00~120.00,0.20~40.00μg.mL-1范围内呈良好线性关系（r≥0.9998）;各组分低、中、高浓度平均加样回收率在98.5%~102.0%之间,RSD（n=3）≤2.3%;重复性良好,RSD（n=6）≤1.6%。结论：本法简便、准确可靠,可用于同时测定挥发油中苍术酮、β-桉叶醇、白术内酯Ⅰ、Ⅲ、β-榄香烯的含量。
Objective：To develop an HPLC method to determine atractylon,β-eudesmol,atractylenolidⅠ,atractylenolidⅢ and β-elemene in Atractylodes macrocephala Koidz volatile oil.Method：The method was carried out by Hypersil ODS2 column（250 mm×4.6 nm,5 μm）,using acetonitrile（A）-water（B） 60∶40 as mobile phase with gradient elution（0-30 min,40%B→10%B;30-40 min,10%B→10%B）,at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 200 nm（β-eudesmol,atractylenolidⅢ）（0-9 min）,220 nm（atractylenolidⅠ）（9.1-20 min） and 200 nm（atractylon,β-elemene）（20.1-45 min）.The column temperature was 30 ℃.Results：The linear relationship was good for atractylon in the range of 0.100-1.000 mg·mL-1,and for β-eudesmol,atractylenolid Ⅰ,atractylenolid Ⅲ and β-elemene in the range of 20.00-1000.00,3.00-150.00,4.00-120.00 and 0.20-40.00 μg·mL-1（r≥0.9998）.The average recoveries（n=3）of the five investigated compounds at low,medium and high concentration levels were in the range of 98.5%-102.0%（RSD≤2.3%）.The repeatability（n=6）were good（RSD≤1.6%）.Conclusion：The method was simple,accurate and reliable.It could be used to determinate the contents of atractylon,β-eudesmol,atractylenolidⅠ,atractylenolid Ⅲ andβ-elemene in volatile oil.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第6期1106-1110,共5页
Chinese Journal of Pharmaceutical Analysis
基金
山西省卫生厅科技攻关项目(200833)
