摘要
目的:研究Tet对缺氧大鼠大脑皮层神经元NMDA受体通道变化的影响。方法:细胞贴附膜片箝法。用95％N_2+5％CO_2饱合无糖浴液产生细胞缺氧模型。结果:在缺氧状态下,35pS通道开放时间常数τ_2,35和100pS通道开放概率增加,Tet 7.5μmol·L^(-1)降低缺血所致35和100 pS通道开放概率,15和30μmol·L^(-1)完全抑制100pS通道,改变35pS的开放时间常数为单指数分布。结论:抑制缺氧所致NMDA受体通道开放是Tet对受缺氧损伤大脑保护作用的机制之一。
AIM: To study the effects of tetrandrine (Tet) on the changes of NMDA receptor channels in cortical neurons induced by anoxia. METHODS: Cell-attached configuration of patch-clamp techniques. Anoxia was produced by perfused cells with 95 % N2+5 % CO2 gassed bath solution. RESULTS: During anoxia, the open time constant (τ2), open probability (Po) of 35-pS and 100-pS channels increased. Tet 7.5 μmol·L-1 reduced the Po of 35-pS and 100-pS channels, 15 and 30 μmol·L-1 inhibited open of 100-pS channel fully, and changed the open time constant of 35-pS from two to single exponential distribution. CONCLUSION: Tet inhibition of the open of NMDA receptor channels induced by anoxia was one of its protective mechanisms.
出处
《中国药理学报》
CSCD
1999年第8期729-732,共4页
Acta Pharmacologica Sinica
关键词
粉防已碱
NMDA受体
离子通道
膜片箝技术
药理
tetrandrine
N-methyl-D-aspartate receptors
ion channels
patch-clamp techniques
cerebral cortex
anoxia
dizocilpine maleate
