摘要
目的:采用近红外光谱法对消渴丸浓缩液中葛根素含量进行快速测定。方法:以HPLC分析值作为参照,采用近红外透射光谱技术和光纤探头采集消渴丸浓缩液的近红外光谱,结合偏最小二乘法建立葛根素含量的快速测定方法,并对未知样品进行了含量预测。结果:建立的葛根素校正模型R2,RMSECV,RPD值分别为0.955 9,0.241,4.36。对预测集样品预测平均相对偏差为1.8%。结论:该方法准确、快速、简便,可作为消渴丸提取浓缩过程质量检测和实时在线质量控制的方法。
Objective：To determine the content of puerarin in the concentrating process of Xiaoke pills rapidly by near-infrared spectroscopy.Method：Near-infrared transmissionspectroscopy technology and fiber optic probe were used to collect NIR spectra of Xiaoke pills concentration liquid.With HPLC analysis results as reference,the fast determination method of puerarin was established with partial least squares（PLS）.Result：The R2,RMSECV and RPD of the calibration model for puerarin were 0.955 9,0.241 and 4.36,respectively.The average relative deviation of the predication set was 1.8%.Conclusion：The method is accurate,fast and simple,which could be generalized to the on-line quality control of Xiaoke pills concentrating process.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第11期48-51,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(2009ZX09308-003)
关键词
近红外光谱法
消渴丸
葛根素
浓缩液
near-infrared spectroscopy
Xiaoke pill
puerarin
concentrating process
