体外释放酶联免疫法检测结核杆菌γ-干扰素的实验研究被引量：14

Experimental research on the detection of MTB IFN-γ in vitro release by ELISA

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摘要目的:研究体外释放酶联免疫法检测结核分枝杆菌相关γ-干扰素定量试验对结核病的诊断价值。方法:收集2008年7月-2010年10月来盐都区疾病控制中心结核病诊疗门诊就诊的肺结核、疑似肺结核及肺外结核患者共883人,采用体外释放酶联免疫法定量检测结核分枝杆菌相关γ-干扰素,判断是否感染结核分枝杆菌。结果:TB-IGRA在痰菌涂片阳性肺结核、临床诊断肺结核、肺外结核病例中检出率分别为89.11%、84.48%、85.71%,检测结果的特异性为91.57%。结论:TB-IGRA检测可作为结核分枝杆菌感染诊断的一种常规实验方法,且TB-IGRA的动态检测对抗结核治疗效果具有较好的评价作用。 Objective：To detect mycobacterium tuberculosis（MTB） related interferon-gamma（IFN-γ） in vitro release by Enzyme-linked immunosorbent assay,and evaluate the diagnosis value of the quantitative test against tuberculosis（TB）.Methods：883 outpatients with active TB,suspected TB and extrapulmonary tuberculosis were recruited from Yandu district CDC from July 2008 to Oct 2010,mycobacterium tuberculosis（MTB） related interferon-gamma（IFN-γ） in vitro release was detected quantitatively by Enzyme-linked immunosorbent assay,and to judge the infection status of TB.Results：The detection rate of TB-IGRA in active TB patients with positive sputum smear was 89.11%,while the rates in clinically diagnosed TB patients and extrapulmonary tuberculosis patients were 84.48%,85.71% respectively.The test specificity was 91.57%.Conclusion：TB-IGRA can be used as a routine experimental tool for diagnosis of mycobacterium tuberculosis infection,and its dynamic surveillance can evaluate the effect of anti-tuberculosis therapy well.

作者王荣堂陈春梅朱晓华葛胜祥王剑甦张宏兰袁中杭符美娟

机构地区江苏省盐城市盐都区疾病预防控制中心浙江大学国家传染病诊断试剂与疫苗工程技术研究中心

出处《中国卫生检验杂志》 CAS 2011年第5期1163-1164,1166,共3页 Chinese Journal of Health Laboratory Technology

基金国家科技重大专项课题资助项目(2008ZX10003-004)

关键词体外释放酶联免疫法 Γ-干扰素结核分枝杆菌 Vitro release ELISA IFN-γ Mycobacterium tuberculosis

分类号 R52 [医药卫生—内科学]

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