摘要
目的考察2种血小板滤器滤除手工法制备的浓缩血小板中的白细胞后血小板功能的变化情况。方法采用富血小板血浆法(PRP法)以400 ml新鲜全血制备浓缩血小板,将6袋ABO同型的浓缩血小板汇集,并用2种国产血小板型去白细胞滤器过滤,各10例(分别以A、B组代之),测定过滤前后的血小板计数、白细胞计数、pH值、血小板CD62p阳性表达率、血小板聚集和低渗休克等指标。结果血小板去白过滤后,A、B 2种滤器(组)的血小板回收率、剩余白细胞数及pH值分别为(87.01±3.47)%vs(87.88±4.77)%、(0.95±0.90)×106vs(0.45±0.58)×106及(7.13±0.13)vs(6.80±0.26)(P>0.05);血小板过滤前后CD62p阳性表达率、血小板最大聚集率和低渗休克,A组分别为(8.06±4.11)%vs(8.21±4.50)%、(70.55±27.21)%vs(71.63±32.24)%和(68.14±10.13)%vs(69.18±9.38)%,B组分别为(10.34±3.26)%vs(10.47±2.42)%、(56.30±18.43)%vs(59.49±19.15)%和(75.73±5.50)vs(73.74±6.52)%(P>0.05)。结论所考察的2种血小板型去白细胞滤器过滤浓缩血小板未增加血小板的活化,对血小板聚集功能及抗低渗休克能力无明显影响,血小板回收率及剩余白细胞数符合相关标准。
Objective To evaluate the changes in platelet function after filtering leukocytes with two types of leukocytes filter for PCs.Methods PCs was prepared from 400 ml fresh whole blood by platelet-rich plasma（PRP） method.Six bags of ABO-matched PCs were pooled,and then filtered with two types of domestic filters（namely group A and B）,10 samples respectively.The counted numbers of platelet and leukocyte,pH,CD62p expression（%）,platelet aggregation and hypotonic shock response（HSR） were detected before and after filtering.Results After filtering leukocytes,the platelet recovery,the residual leukocytes and the pH,group A and B respectively,were（87.01±3.47）% vs（87.88±4.77）%,（0.95±0.90）×106 vs（0.45±0.58）×106 and（7.13±0.13） vs（6.80±0.26）.The CD62p expression（%）,platelet aggregation and hypotonic shock response（HSR） of group A before and after filtering were（8.06±4.11）% vs（8.21±4.50）%,（70.55±27.21）% vs（71.63±32.24）% and（68.14±10.13）% vs（69.18±9.38）%,while those of group B were（10.34±3.26）% vs（10.47±2.42）%,（56.30±18.43）% vs（59.49±19.15）% and（75.73±5.50） vs（73.74±6.52）%,respectively.There were no significant difference（P0.05）between these items before and after filtering.Conclusion Filtration with two types of domestic leukocyte filters didn′t activate platelet and had no influence on platelet aggregation and HSR.Besides,both the platelet recovery and residual leukocytes met the requirements of Chinese standards.These two types of domestic leukocyte filters could be applicable to the leukocyte removing for PCs.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2011年第4期323-326,共4页
Chinese Journal of Blood Transfusion
基金
四川省科技支撑计划(2008SZ0101)
