摘要
目的探讨血管内皮生长因子（VEGF）和转化生长因子-α（TGF-α）在尖锐湿疣（CA）血管形成中的作用。方法用RT—PCR和免疫组化法检测30例CA和15例正常包皮中VEGF、TGF-α和CD34表达。结果CA中VEGF和TGF—dmRNA表达明显高于正常包皮（P〈0．001）；CA中VEGF、TGF-α蛋白阳性表达率分别为90％、86．7％，正常包皮中则为40％、26．7％，二者的差异具有统计学意义（P〈0．01）。CA中微血管密度比正常包皮明显增多（P〈0．01），且CA中VEGF、TGF-α蛋白表达与MVD呈显著正相关（P〈0．01）。结论CA中VEGF和TGF-α表达上调，可能促进血管生成，参与CA的发生、发展。
Objective To investigate the role of vascular endothelial growth factor （VEGF） and transforming growth factor-ct （TGF-α） in the angiogenesis of eondyloma acuminatum （CA）. Methods Tissue specimens were obtained from the lesions of 30 patients with CA and foreskin of 15 normal human controls. Reverse transcription （RT）-PCR and immunohistochemical method were utilized to measure the protein and mRNA expressions of VEGF and TGF-α in the specimens. Mierovessel density （MVD） was determined by staining with anti-CD34 polyelonal antibodies. Results A statistical elevation was observed in the expression of VEGF and TGF-α mRNA as well as MVD in CA specimens compared with the normal control specimens （P 〈 0.001, 0.001, 0.01）. The expressions of VEGF and TGF-α protein were observed in 90% and 86.7% of the CA specimens, and in 40% and 26.7% of the control specimens （both P 〈 0.01 ）. MVD was positively correlated with the expression of VEGF and TGF-α protein in CA tissues （both P 〈 0.01 ）. Conclusion The overexpression of VEGF and TGF-α in CA tissue may accelerate angiogenesis in, and participate in the development and progression of, CA.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2011年第5期350-351,共2页
Chinese Journal of Dermatology
基金
广东省2005年（财政）科技三项基金
