摘要
本研究目的为了探讨环孢霉素A（CsA）对人早孕期母-胎界面主要组成细胞产生的IL-10与IFN-γ比率的调节作用,为治疗复发性自然流产等妊娠相关性疾病提供新线索。收集6~10周行人工流产术的正常妊娠妇女的绒毛和蜕膜组织;体外分离、培养获得蜕膜免疫细胞、蜕膜基质细胞与滋养细胞,建立三种细胞之间的两两共培养和三者共培养模型;ELISA法检测经环孢素A（0μmol/L、1μmol/L）处理48 h的共培养体系培养上清中IL-10与IFN-γ分泌水平。结果显示,（1）1μmol/L CsA可以明显促进两两共培养（P〈0.05）,尤其三种细胞共培养体系IL-10的分泌（P〈0.01）;相反,抑制蜕膜免疫细胞与滋养细胞或蜕膜基质细胞两两共培养（P〈0.05）,及三者共培养体系中IFN-γ的产生（P〈0.05）;（2）低剂量CsA升调两两共培养和三者共培养体系中IL-10与IFN-γ比率（P〈0.01）。结果表明,CsA通过促进母-胎界面IL-10/IFN-γ的比率,从而有利于母-胎界面Th2型免疫优势的维持。
To explore the regulatory effect of cyclosporine A（CsA） on the ratio of IL-10 to IFN-γ at the materno-fetal interface during human first trimester,which would provide evidence to treat pregnancy wastage and other pathological pregnancy.human placental and decidual tissues were collected in artificial abortion at 6～10 weeks of gestation.The decidual immuno-competent cells,trophoblast cells and decidual stromal cells were isolated by proteinase digestion and co-cultivation in vitro,and the supernatants were harvested respectively from the co-cultured cells after treatment with 1 μmol/L CsA or without CsA for 48 h.While the level of IL-10 and IFN-γ in the culture supernatants was measured by ELISA.It was found that CsA of 1μmol/L could significantly promote the secretion of IL-10 and inhibit secretion of IFN-γ in the co-cultivation of the functional cells at the maternal-fetal interface.The ratio of IL-10 to IFN-γ was significantly increased after treatment with CsA,particularly in the co-culture of all functional cells.These results suggest that CsA can improve the Th2 bias at the maternal-fetal interface in human first-trimester pregnancy.
出处
《现代免疫学》
CAS
CSCD
北大核心
2011年第2期121-125,共5页
Current Immunology
基金
国家重大科学研究计划项目(2006CB944007)
国家自然科学基金重点资助项目(30730087)
面上项目(30872768
81070537)
上海市医学领军人才基金项目
