摘要
按照毕氏酵母的偏爱密码子,设计并合成了海葵毒素AP-b的基因序列,将合成基因序列通过一系列操作导入毕氏酵母表达载体pPICZα中,以电穿孔方法转化毕氏酵母菌株X33,通过PCR法鉴定,筛选出能够表达重组海葵毒素的酵母菌株.结果表明,构建出海葵毒素AP-B毕赤酵母真核表达体系,并鉴定出4株含有海葵毒素基因的毕赤酵母工程菌.
Synthesis of Ap-B gene sequence which was inserted into pPICZαvector was designed by code-modifications of pastoris.Transformed pPICZαvector into Pichia pastoris X33 via electroporation.Then Expression System was Identificated by PCR.The transformed Pichia pastoris screened by using the expression primers.The resurt show that our studies succeed in secreted expression system of rAp-B in Pichia pastoris and 4 strains of engineered bacteria identified.
出处
《内蒙古民族大学学报（自然科学版）》
2011年第2期180-183,共4页
Journal of Inner Mongolia Minzu University：Natural Sciences
