摘要
采用连续２次ＦｉｃｏｌＨｙｐａｑｕｅ分离，初步纯化脐血造血细胞，将其单个核细胞接种于经γ射线预处理的成人骨髓基质细胞层上，加入适量ＩＬ－３，并设不加ＩＬ－３的对照组，持续培养６周，定期检测非粘附细胞和集落形成细胞（ＣＦＣ）。结果：ＩＬ－３组３～６周非粘附细胞数和ＣＦＣ产率显著高于对照组，分别为对照组的２．７～４．５倍和１．６～２．３倍。
To elucidate the effect of IL3 on the proliferation and differentiation of cord blood hematopoietic cells in vitro.To preliminarily purify cord blood hematopoietic cells by continuiously double FicollHypaque method.The adult marrow stromal layer was established by static liqued culture,then irradiated with ray.The mononuclear cells(MNCs) were cultured in stromalcontact system with or without IL3 for six weeks.The nonadherent cells and CFCs were counted and measured at different interval.Results: In the experimental group of IL3 the number of nonadherent cells (2.74.5 fold increase )and the production of CFCs(1.62.3 fold increase) were remarkably greater than that in the control group.IL3 enhanced the proliferation and differentation of primitive hematopoietic cells from cord blood in the stomasupported culture.
出处
《苏州医学院学报》
1999年第7期756-757,783,共3页
Acta Academiae Medicinae Suzhou
