摘要
目的检测高压电对大鼠血清肿瘤坏死因子-α(TNF-α)、皮肤微循环灌流量(SMH)的影响并探讨TNF-α在微循环障碍中的作用及乌司他丁(UTI)的干预效果。方法将144只SD大鼠按完全随机设计方法分为对照组、电伤组和治疗组,每组48只,每组再分为6个时相组,每时相组8只。电伤组和治疗组大鼠用调压器和实验变压器制成高压电烧伤模型,对照组大鼠接相同装置但不通电,致假伤。用ELISA法检测三组伤前15 m in及伤后5 m in、1 h、2 h、4 h、8 h大鼠血清TNF-α变化,用激光多普勒微循环图像仪检测以上各时相胸部SMH。结果①TNF-α变化:组内比较,电伤组和治疗组TNF-α在伤后5 m in上升,持续至伤后8 h,均呈逐渐升高趋势,伤后8 h达最高值,分别为(54.71±4.82)、(24.26±3.17)pg/m l;组间比较,治疗组TNF-α在伤后5 m in较电伤组无明显变化,从伤后1~8 h,治疗组TNF-α均低于电伤组,高于对照组。②SMH变化:组内比较,电伤组和治疗组SMH在伤后5 m in开始下降,持续到伤后8 h,均呈逐渐下降趋势,均以伤后5 m in最低值,分别是(1.01±0.05)V、(1.12±0.11)V;组间比较,治疗组SMH在伤后5 m in^8 h,SMH均高于电伤组,低于对照组。结论高压电可引起大鼠血清TNF-α升高及SMH下降,而UTI能抑制伤后血清TNF-α升高和SMH下降。TNF-α在高压电烧伤后微循环障碍中起一定作用。
Objective To observe the influence of high-voltage electricity on rats’ tumor necrosis factor（TNF-α） and skin microcirculation hemoperfusion（SMH）,to explore the effect of TNF-α on microcirculation disturbance and the therapeutic effects of Ulinastatin（UTI）.Methods One hundred forty four SD rats were divided into control group,electrical burn group and UTI treatment group in a simple random method,with forty eight rabbits in each group,each group was divided into six phases.Rabbit model of high-voltage electrical burn（HEB） both electrical burn group and UTI treatment group were reproduced with voltage regulator and transformer.The control group was not electric shock.Enzyme linked immunosorbent assay（ELISA） methods was used to check out three groups TNF-α at 15 minutes before HEB,5 minutes,1 hour,2 hours,4 hours,8 hours after HEB.The LISCA Laser Doppler Hemoperfusion image instrument（LDPI） was used to measure the hemoperfusion of chest skin.Results ①The alteration of TNF-α,comparison within the group,TNF-α values increased at 5 minutes after HEB both UTI treatment group and electrical burn group,and it increased gradually to 8 hours after HEB,the peak values were（54.71±4.82）,（24.26±3.17）pg/ml;comparison TNF-α values at the same time intervals between the three groups,there was no significant difference at 5 minutes after HEB,TNF-α values of UTI treatment group were smaller than those of electrical burn group,but bigger than control group from 1 hour to 8 hours after HEB.②The alteration of SMH,comparison within the group,SMH values decreased at 5 minutes after HEB both UTI treatment group and electrical burn group,and it decreased gradually to 8 hours after HEB,the both SMH values were（1.01±0.05）,（1.12±0.11）V at 5 minutes after HEB.Comparison SMH values at the same time intervals between the three groups,there was no significant difference between UTI treatment group and electrical burn group at 15 minutes before HEB,SMH values of UTI treatment group were bigger than those of electrical burn group,but smaller than those of control group form 5 minutes to 8h after HEB.Conclusion High-voltage can cause rats serum TNF-α rise and SMH decline,but UTI can inhibit the serum TNF-α rise and SMH decline.TNF-α played an important role in microcirculation disturbance after HEB
出处
《中华全科医学》
2011年第5期670-671,680,共3页
Chinese Journal of General Practice
基金
河北省自然科学基金(302551)
关键词
烧伤
电
TNF—α微循环
灌流量
乌司他丁
Electricity burn
TNF-α
Microcirculation
Hemoperfusion
Ulinastatin
