摘要
目的:观察原花青素(PC)对心肌梗死(MI)大鼠炎性浸润及其对肿瘤坏死因子-α(TNF-α)表达的影响。方法:结扎大鼠左冠状动脉前降支构建大鼠MI模型,存活大鼠随即分为MI组和PC组,另设假手术组(Sham组),各组按观察时相2周和4周再分为:MI 2周和4周,PC 2周和4周,sham 2周和4周;组织病理学观察术后2周及4周各组心肌组织病理结构;逆转录聚合酶联反应(RT-PCR)检测TNF-α的mRNA表达,Westernblotting及免疫组织化学检测TNF-α蛋白表达。结果:MI后2周及4周:①与Sham组比较,MI组和PC组心肌组织结构显著异常;与MI组比较,PC组心肌结构明显改善。②与Sham组比较,MI组和PC组TNF-α在基因和蛋白水平上显著增加,差异有统计学意义(P<0.01);与MI组比较,PC组TNF-α在基因或蛋白水平上表达显著减少,差异有统计学意义(P<0.01)。结论:PC能够减轻大鼠MI后的炎性浸润,下调TNF-α表达。
Objective：To investigate the effcts of procyanidin（PC） on rat myocardial inflammatory infiltration and TNF-alpha（tumor necrosis factor-α,TNF-α） expression.Method：The rat myocardial infarction model was established by left anterior descending coronary artery ligation.Survival rats were randomly divided into the myocardial infarction（MI） control group（n=9） and the procyanidin group [PC n=9,100 mg/（kg·d）],and another sham group（n=10） was in the study.Each group further divided into by 2 weeks and 4 weeks： MI 2 w and 4 w,PC 2 w and 4 w,sham 2 w and 4 w;myocardial pathological structure in the infarcted and non-infarcted zone were observed at 2 and 4 weeks after MI with histopathological methods.The expression of TNF-α was detcted by semiquantitative RT-PCR,and the expression of TNF-α was detcted by immunohistochemistry and Western blotting.Result：After MI 2 and 4 weeks,①compared with sham group,major anomalies of myocardial pathological structure in MI and PC groups;compared with MI group,noticeable improvements of myocardial pathological structure in PC group.②compared with sham group,the gene and protein expression of TNFα was higher significantly in MI and PC groups（P0.01）.Compared with MI group,the gene and protein expression of TNF-α was lower significantly in sham and PC groups（P0.01）.Conclusion：PC can attenuate the myocardial inflammatory infiltration and suppress the expression of TNF-α
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2011年第3期208-211,共4页
Journal of Clinical Cardiology
关键词
心肌梗死
原花青素
肿瘤坏死因子
myocardial infarction
procyanidin
tumor necrosis factor-α
