摘要
目的:以经方升降散为基础,加味组成理肺化纤方,研究理肺化纤方对肺纤维化的防治作用以及对肺纤维化大鼠肺功能及血清和组织羟脯氨酸含量的影响。方法:Wistar大鼠100只,体重(200±20)g,雌雄各半,随机分为5组,每组20只。模型组与各治疗组实验大鼠气管内注入博莱霉素A5复制肺纤维化模型,正常对照组和模型组给等容积生理盐水ig、理肺化纤方治疗组给药剂量为4.41 g.kg-1.d-1、强的松对照组给药剂量为1.05 mg.kg-1.d-1、强的松合理肺化纤方治疗组给等量的强的松和理肺化纤方水煎液ig,实验分两批,分别于7,28 d末次给药后,采用Anires2005动物肺功能分析系统测试肺功能和样本碱水解法检测实验大鼠的血清和组织羟脯氨酸(Hyp)的含量。结果:肺组织病理学方法观察苏木精-伊红及Masson染色切片,观察显示造模成功,检测实验大鼠的肺功能用力肺活量(FVC)、最大呼出量(PEF)和第0.25 s用力呼出容积占用力肺活量百分比(FEV 0.25/FVC%)、最大通气量(MVV)等指标表明各治疗组与模型组对比肺功能和血清及组织Hyp含量均有显著改善,以中西药结合组效果更为明显。结论:理肺化纤方能明显改善肺纤维化大鼠的肺功能和降低血清及组织Hyp含量,尤其在加用激素强的松治疗时能明显减轻肺纤维化大鼠的肺纤维化程度。
Objective：To study prevention and treatment of pulmonary fibrosis by Lifei Huaxian prescription（LHP）,a formulated prescription based on traditional prescription Shengjiang San.And to investigate its influence on pulmonary function and the content of hydroxyproline in serum and in tissue.Method： One hundred Wistar rats（half male and half female）,weighing（200 ± 20）g,were randomly divided into 5 groups evenly（20 in each）.Model of pulmonary fibrosis was established by injection of amphotericin A5 in the airway.Normal controls and model group were given the same volume of physiological saline.The dosage of LHP was 4.41 g.kg-1.d-1,prednisone dosage was 1.05 mg.kg-1.d-1,prednisone ＋ LHP treatment group was given the same amount of prednisone and LHP.The experiment lasted for 7,28 d.Afterwards,animal lung function was analyzed and the serum and lung tissue hydroxyproline content was determined.Pathological method was used for morphological observation with sappanwood fine-Iraq red and Masson dyeing slice.Result： The lung tissue morphology showed that the model establishment was successful.The lung function of forced vital capacity（FVC）,largest exhaled quantity（PEF） and the first 0.25 s hard exhaled volume percentage of forced vital capacity（FEV 0.25 /FVC%）,maximum volume（MVV） were investigated.Testss showed that the treatment group and model group had significant differences in lung function and serum and tissue hydroxyproline content.They were significantly improved by the combination of LHP and prednisone.Conclusion： Lifei Huaxian prescription can significantly improve pulmonary fibrosis and lung function in rats with pulmonary fibrosis,and reduce serum and tissue hydroxyproline content,especially when applied with prednisone treatment.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第9期194-198,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
甘肃省卫生厅中医药科研项目(甘科鉴字[2009]043号)
关键词
理肺化纤方
肺纤维化
肺功能
羟脯氨酸
Lifei Huaxian prescription
pulmonary fibrosis
lung function
hydroxyproline
