摘要
目的:研究筛检海产品中副溶血弧菌方法,提高检出率和检测速度。方法:用PCR快速筛检,GB法寻找目标菌,以生化分步筛检作出初步确定,再经系统生化鉴定确认之。结果:356份海产品经筛检检出副溶血弧菌141株,检出率为39.61%,其中PCR一次性检出了141株副溶血性弧菌阳性基因,GB法分离到115株副溶血性弧菌,26株通过二次增菌才分离到,生化筛检与系统特殊化一致率达100%,检出带TDH毒力基因副溶血性弧菌5株,未检出TRH毒力基因。结论:应用发现PCR、GB和典型生化分步联合的筛检方法,可用于筛检食品中副溶血弧菌,具有快捷、大容量、方便的优点,为进一步开展食品多病原污染物监测工作打下了基础。
Objective：To establish a method to enhance the detection capacity and efficiency of Vibrio parahaemolyticus（VP） in seafood.Methods：PCR method was used firstly to do the rapid screening,and then Chinese Standardized method（GB method）,to identify the target bacterium.Systematic biochemical specialization was used to final identifying.Results：141 VP strains were screened positive in 356 seafood samples,positive rate was 39.61%.All 141 positive strains were detected by PCR method at a time,while the GB method,115 positive strains were detected by primary culture and 26 others were found by cultured in secondary enrichment medium.The Consistent rate between the biochemical screening method and systematic biochemical specialization method reached 100%.5 strains with TDH virulence was found,while no strain with TRH virulence was detected.Conclusion：The combination of PCR,GB with other typical biochemical method can be used for detecting VP in seafood,which is quick,convenient and effective.The method could be the base for monitoring multi-pathogens pollutant in food.
出处
《中国卫生检验杂志》
CAS
2011年第3期639-641,共3页
Chinese Journal of Health Laboratory Technology
关键词
副溶血弧菌
海产品
筛检方法
毒力基因
Vibrio parahaemolyticus
Seafood
Screening method
Virulence genes
