摘要
目的:建立HPLC和TLC测定复方葛根胶囊中葛根素含量方法,并进行比较分析。方法:色谱条件:色谱柱为C18柱,流动相为甲醇-0.1%磷酸溶液(25:75),检测波长250nm。TLC:硅胶GF254薄层板展开,甲醇-乙酸乙酯-石油醚-水(9:3:2:0.2)为展开剂。结果:建立HPLC、TLC测定葛根素的方法;最小检出限分别为0.05、0.1μg/mL;HPLC在24h内稳定性良好,TLC在8h内稳定性良好。结论:两种方法可行,HPLC灵敏度高,TLC一定条件下代替HPLC作为葛根素含量快检的方法效果更好。
Objective：To establish methods for determining Puerarin by HPLC and TLC.Methods：For HPLC：C18 column,the flow rate was l mL/min and wave was set at 250 nm.For TLC ：silica gel was GF254 and a mixture as mobile phase.Results：Establish both methods,the limits of detection were 0.1μg/mL and 0.05μg/mL,stability were 24h and 8h for TLC and HPLC.Conclusion：Both of the methods are feasible,each has its advantages.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第4期695-697,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(No.30572297)
山西省国际科技合作计划项目(No.2009081038)
"十二五"国家科技支撑计划(No.2011BAI07B05)
太原市重点成果转化科技项目(No.20110602)~~
