摘要
目的了解成都市乙型肝炎病毒(HBV)基因型分布特点,探讨HBV基因型与疾病临床表现的相关性。方法随机选择成都市HBV感染者422例,采用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)鉴定HBV基因型。结果 HBV/B基因型366例占86.7%(366/422),HBV/C基因型53例占12.6%(53/422),HBV/D基因型3例占0.7%(3/422)。B、C基因型感染者相比,HBeAg、抗-HBeAg阳性率及HBV DNA水平的差异均无统计学意义(P>0.05),C基因型感染者更易发生肝硬化(P=0.004)及肝细胞癌(P=0.029)。结论成都市HBV感染者所携带的HBV存在B、C、D 3种基因型,以B型为主,其次C型,D型最少;C基因型感染者更易发生较严重的肝脏疾病,如肝硬化及肝细胞癌。
[ Objectives] To investigate the distribution of hepatitis B virus genotype in Chengdu and to discuss the relationship between HBV genotypes and its clinical manifestations. [Methods] Serum samples from 422 patients with chronic HBV infection were determined for HBV genotypes by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. [Results] Of the 422 samples, 86.7% （366/ 422） were genotype B and 12.6% （53/422） were genotype C, 0.7% （3/422） were coinfected by genotype D. Compare with genotype B and C, there were no statistical diferences in the rate of positive in HBeAg, anti- HBeAg ＆ HtlV DNA level. However, genotype C was more prevalent in the LC and HCC patients. [Conclusions] These results indicate genotype B, C and D exist in our area, the dominant genotype of HBV is genotype B, then genotype C, genotype D is the least. Genotype C maybe associated with development of severe liver diseases including LC and HCC.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2011年第5期575-578,共4页
China Journal of Modern Medicine
基金
成都医学院创新性实验项目(2008)
成都医学院自然基金项目(No:CYZ08-015)
关键词
乙型肝炎病毒
基因型
分布
hepatitis B virus
genotype
distribution
