摘要
以69个玉米自交系为试材,在适宜耐盐碱筛选的2个浓度水平(25 mmol/L的Na2CO3、100 mmol/L的NaCl)下,进行种子萌发和幼苗胁迫试验,分别测定发芽率、相对电导率、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量等适宜耐盐碱筛选的5项生理生化指标,进而转化为耐盐碱系数,用以比较供试自交系的耐盐碱能力。结果表明:(1)多数材料在胁迫条件各指标变化趋势相同;(2)材料间耐盐碱能力差异显著;(3)筛选出高耐盐碱自交系5份,耐盐碱自交系15份,中度耐盐碱自交系32份,敏感自交系10份,高度敏感自交系7份。研究结果将为玉米耐盐碱种质资源筛选提供试验依据。
Under alkali-stress condition（25 mmol/L Na2CO3） and salt-stress condition（100 mmol/L NaCl）,sixty-nine maize inbred lines were cultivated.The five appropriate physiological and biochemical indicies were tested and used to evaluate salt-alkali tolerance of inbred lines,which included germination percentage,relative conductivity,SOD activity,MDA content and proline content.The main results were summarized as follows：（1）most of these materials had the same trends for five indicies in stress conditions;（2）salt-alkali tolerance between materials had obvious difference;（3）5 inbred lines with high salt-alkali tolerance,15 inbred lines with salt-alkali tolerance,32 inbred lines with medium salt-alkali tolerance,10 inbred lines with salt-alkali susceptible,7 inbred lines with high salt-alkali susceptible.The results would provide reference for screening salt-alkali tolerance germplasms.
出处
《种子》
CSCD
北大核心
2011年第3期1-6,共6页
Seed
基金
2009年农业部转基因重大专项“抗逆转基因玉米新品种培育”(2009ZX08003-018B)
2009年农业部转基因重大专项“通过基础抗性与诱导抗性基因的协同操纵进行抗旱转基因玉米新品种培育”(2009ZX08003-009B)资助
关键词
玉米
自交系
盐碱胁迫
maize
inbred lines
saline-alkali stress
