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透射比浊法和散射比浊法测定血清胱抑素C的方法学评价 被引量:9

Methodological evaluation of transmission and scattering turbidimetry for the detection of serum cystatin C
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摘要 目的探讨同一临床实验室不同分析系统之间检测血清胱抑素C的结果是否具有可比性。方法以散射比浊为比较方法,透射比浊为试验方法,分别检测40例患者新鲜血清,记录检验结果,去除离群值,计算回归方程,评估两分析系统的预期偏倚和相对偏倚。结果血清胱抑素C透射比浊法和散射比浊法测定结果的相关回归方程为Y=1.011X+0.13(r2=0.992 3)。结论血清胱抑素C透射比浊法和散射比浊法测定结果的预期偏倚在可接受范围内。 Objective To investigate the comparability level for the results of serum cystatin C measured by two different detection systems using transmission and scattering turbidimetry respectively through method comparison and bias estimation. Methods Forty fresh serum samples were measured using scattering turbidimetry, which was the comparative method and transmission turbidimetry, which was the experimental method. Method comparison and bias estimation were done, and the anticipated and relative bias was evaluated based on the CLSI EP9-A documents. Results The regression equation of serum cystatin C measured by transmission and scattering turbidimetry was Y= 1. 011X+0.13. The relative coefficient was 0. 996 1 and the determination coefficient was 0. 992 3. Conclusion The anticipate bias of the result of serum cystatin C measured by transmission and scattering turbidimetry is acceptable.
出处 《国际检验医学杂志》 CAS 2011年第3期340-340,368,共2页 International Journal of Laboratory Medicine
关键词 散射测浊法和比法 偏倚(流行病学) 胱抑素C nephelometry and turbidimetry bias(epidemilogoy) cystatin C
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