摘要
目的:探讨新疆地区维吾尔族(维族)、汉族妇女宫颈病变脱落细胞中人类染色体端粒酶基因(human telomerase gene,hTERC)的表达差异及其与人乳头瘤病毒(human papillomavirus,HPV)高危亚型感染的相关性。方法:采用荧光原位杂交技术,比较50例维族和52例汉族宫颈病变患者hTERC基因扩增情况,同时检测HPV感染情况。结果:维族宫颈病变患者hTERC基因扩增率为62.00%,汉族宫颈病变hTERC基因扩增率为51.92%。随病变进展,维、汉族宫颈病变患者hTERC基因扩增均明显增加。维、汉族宫颈癌hTERC基因平均扩增倍数,在HPV各亚型感染组间差异无统计学意义(P>0.05),但在HPV多重感染和单一感染间差异有统计学意义(P<0.05)。维族宫颈病变总hTERC基因扩增倍数与汉族差异有统计学意义(P<0.05)。结论:hTERC基因扩增与高危型HPV感染有关,可能是宫颈癌进展的预测指标;多重HPV高危亚型感染可能是维族宫颈癌发病率高的原因之一。
Objective To investigate the difference of human telomerase gene amplification in cervical exfoliated cells between Uygur and Han patients with cervical diseases,and their relationship with high-risk subtypes of human papillomavirus infection.Methods Fifty Uygur and 52 Han patients with cervical diseases were detected with fluorescence in situ hybridization to compare the amplification of human telomerase gene,and human papillomavirus infection was detected.Results Human telomerase gene amplification rates were 62.00% and 51.92% in Uygur and Han patients with cervical disease.With the progression of disease,the human telomerase gene amplification rate increased significantly.There were significant differences in average human telomerase gene multiples amplification between multiple infection and single infection（P0.05）,and no significant differences among the infections of each human papillomavirus subtype in Uygur and Han patients.However,there was significant difference in the total of human telomerase gene multiple amplification between Uygur and Han patients（P0.05）.Conclusion Human telomerase gene amplification is correlated with high-risk human papillomavirus infection,and it might be a predictor of progression of cervical cancer.Multiple high-risk subtype of human papillomavirus infection may be one of causes of the high incidence of cervical cancer in Uygur patients.
出处
《中华实用诊断与治疗杂志》
2011年第3期238-240,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
新疆医科大学第一附属医院青年专项科研基金资助项目(2009-QN-4)
关键词
宫颈上皮内瘤样病变
人类染色体端粒酶基因
荧光原位杂交
人乳头瘤病毒
维吾尔族
汉族
Cervical intraepithelial neoplasia
human telomerase gene
fluorescence in situ hybridization
human papillomavirus
Uygur nationality
Han nationality
