摘要
目的探讨鼠神经生长因子(NGF)对新生大鼠缺氧缺血性脑损伤(HIBD)后神经修复的作用。方法新生7日龄(SD)大鼠72只随机分成假手术组、模型组、治疗组,模型组腹腔注射生理盐水,治疗组腹腔注射NGF,采用免疫组化检测术后不同时间点(3 h、12 h、1 d、3 d、7 d、14 d)血管内皮生长因子(VEGF)及神经丝蛋白(NFP)的表达。结果假手术组VEGF呈持续的低程度表达,HIBD后治疗组各时间点VEGF的表达均高于模型组,3 h^3 d治疗组的VEGF阳性区平均积分光密度(IOD)值大于模型组(P<0.05),且高峰提前至3 h,2组间7 d及14 d VEGF表达无统计学差异。HIBD后,3 h^1 d模型组和治疗组的NFP阳性区平均IOD值均显著低于假手术组(P<0.01),3 d、7 d及14 d治疗组NFP阳性区平均IOD值显著高于模型组(P<0.01)。结论 NGF对HIBD的神经修复机制之一,可能是通过上调VEGF及NFP的表达而实现。
ObjectiveTo investigate the effects of mouse nerve growth factor（NGF） on neural repair after neonatal rats with hypoxic-ischemic brain damage（HIBD）.MethodsSeventy-two seven-day-old Sprague-Dawley（SD） rats were randomly divided into three groups： the sham group,model group and treatment group.The model group was injected intraperitoneally with normal saline solution,and the treatment group with NGF.Immunohistochemistry was used to investigate the expression of vascular endothelial growth factor（VEGF） and neurofilament protein（NFP） at different time points（3 h,12 h,1 d,3 d,7 d,14 d） after the operation.ResultsThe expression of VEGF in the sham group was maintained at a low level compared to the model group and treatment group.At different time points after HIBD,the expression of VEGF in the treatment group was higher than that in the model group.The average integrated optical density（IOD） of VEGF-positive area in the treatment group was higher than that in the model group from 3 h to 3 d（P0.05）,and the time to peak was 3 hours;there were no significant differences at 7 d or 14 d time point.The average IOD of NFP-positive area in the model group and treatment group was significantly lower than that in the sham group from 3 h to 1 d（P0.01）,but it was significantly higher in the treatment group than that in the model group at 3 d,7 d and 14 d time points（P0.01）.ConclusionAs one of the mechanism,NGF could promote the nerve function recovery after HIBD by increasing the expression of VEGF and NFP.
出处
《中国实用神经疾病杂志》
2011年第3期15-17,共3页
Chinese Journal of Practical Nervous Diseases
关键词
神经生长因子
缺氧缺血性脑损伤
血管内皮生长因子
神经丝蛋白
Nerve growth factor
Hypoxic-ischemic brain damage
Vascular endothelial growth factor
Neurofilament protein
