摘要
【目的】探讨长白赤松土壤酶活性对大气CO2浓度升高的响应规律。【方法】采用1.2m×0.9m×0.9m的开顶箱控制CO2浓度,试验设高浓度CO2处理(CO2浓度分别为700,500μmol/mol)及开顶箱对照(CK)和裸地对照(B),2个对照处理的CO2浓度均为大气CO2浓度(370μmol/mol)。采用多点混合法于7,8,9月的中旬采集各处理0～10cm土层土壤样品,分析土壤水解酶和氧化还原酶活性的变化规律。【结果】与2个对照处理相比,高浓度CO2条件下,土壤脲酶、淀粉酶、转化酶、过氧化氢酶、多酚氧化酶以及脱氢酶活性均升高,而土壤蛋白酶和磷酸酶活性总体上表现出降低,且不同高浓度CO2处理对土壤蛋白酶和磷酸酶活性的影响差异不显著。【结论】高浓度CO2处理下,长白赤松土壤脲酶、转化酶、淀粉酶、过氧化氢酶、多酚氧化酶以及脱氢酶活性明显增加,而土壤蛋白酶和磷酸酶的活性明显降低;各土壤酶活性的月动态规律在不同程度上受到了高浓度CO2的影响。
[Objective] The study explored the mechanism of effects of elevated CO2 on soil enzyme activities associated with Pinus sylvestriformis seedlings.[Method] OTCs（1.2 m×0.9 m×0.9 m） were used to control elevated CO2（700 μmol/mol and 500 μmol/mol） during the growing season for Pinus sylvestriformis seedlings.At the same time,ambientCO2-chamber amd ambient were also set up.Soil samples（0-10 cm） from different sites were collected to be mixed in the middle ten days of a month in summer.And varying dynamic rules of soil hydrolase and oxidoreductase activities to elevated CO2 were determined.[Result] Soil amylase,invertase,urease,catalase,dehydrogenatase,and polyphenol oxidase activities increased under elevated CO2,but protease and phosephatase activities generally decreased compared with those under ambient CO2-chamber and ambient.And effects of different elevated CO2 on soil protease and phosephatase activities were indistinctive.[Conclusion] Soil amylase,invertase,urease,catalase,dehydrogenatase,and polyphenol oxidase activities increased significantly under elevated CO2,and soil protease and phosphatase activities decreased significantly.Meanwhile,the varying dynamic rules of soil enzyme activities between different months were affected by elevated CO2.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2010年第12期87-92,98,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
长安大学中央高校基本科研业务费专项(CHD2009JC144)
中国科学院知识创新工程重大项目(KZCXI-SW-01)
国家重点基础研究发展规划项目(2002CB412502)
关键词
高浓度CO2
长白赤松
土壤酶活性
elevated CO2
Pinus sylvestriformis
soil enzyme activity
