摘要
目的准确确定豚鼠性周期各个阶段。方法利用阴道组织涂片法,对16只12周龄的雌性豚鼠连续20 d进行阴道涂片,镜检,观察阴道涂片中细胞的变化以及豚鼠性周期各阶段的持续时间。结果在发情间期,阴道涂片以白细胞为主;发情前期,以有核上皮细胞为主;在发情期,以角质化的上皮细胞为主;发情后期白细胞中混有部分有核上皮细胞和少量角化上皮细胞。结论阴道组织涂片法是有效区分豚鼠性周期各个阶段的一种简单方法。
Objective To ascertain the characters of each stage of sexual cycle in guinea pigs.Methods Change in vaginal cells was observed for 20 days in 16 female guinea pigs aged 12 weeks by vaginal smear.Results Vaginal cells observed by vaginal smear were with predominance of leucocytes at diestrus;with predominance of stoned epithelia at proestrus,with predominance of keratinized epithelia at estrus,and with predominance of leucocytes mixed with a few leucocytes and keratinized epithelia at metestrus.Conclusion Vaginal smear method could efficiently distinguish different stages of sexual cycle in guinea pigs.
出处
《实验动物科学》
2010年第6期26-28,共3页
Laboratory Animal Science
关键词
豚鼠
性周期
阴道组织涂片
Guinea pig
Sexual cycle
Vaginal smear
