摘要
通过PCR扩增6个不同地区柑橘黄龙病（citrus huanglongbing，HLB）病原茵的核糖体蛋白基因，采用4种不同的限制性内切酶对PCR产物进行限制性片段长度多态性（RFLP）分析；并对核糖体蛋白基因克隆和测序后，使用DNAman和NCBI Blast等软件对基因序列进行比对分析，并通过软件Mega4．1构建其系统发育树．结果表明：6个分离物核糖体蛋白基因的RFLP指纹图谱有所差异，表现出一定的多态性；不同分离物核糖体蛋白基因的核酸序列和氨基酸序列均存在差异，但相互之间同源性很高，其中核酸序列与数据库中亚洲种（CandidatusLiberibacterasiaticus）的同源性高达99％～100％，与非洲种（Ca．L．africanus）的同源性为85％，与美洲种（Ca．L．americanus）的同源性为77％～80％；系统发育分析显示所有的Ca．L．asiaticus亲缘关系极近，归为一个类群，而后与Ca．L．africanus和Ca．L．americanus组成韧皮部杆菌属（CandidatusLiberibacter）一个大的类群．
Ribosomal protein genes of Candidatus Liberibacter asiaticus from six different geographical regions were amplified, and the restriction fragment length polymorphism （RFLP） of PCR products were analyzed. Then, the sequences of ribosomal protein genes were aligned with software DNAman and NCBI Blast, and the phylogenetic tree was constructed. The results showed that the ribosomal protein genes of six isolates had different RFLP fingerprints and showed some polymorphism, and the differences existed in the nucleotide sequences and amino acid sequences of ribosomal protein genes of different isolates, while the homology between each other was very high. Their homology level was from 99.0% to 100% with other Ca. L. asiaticus in GenBank, 85% with Ca. L. africanus, and 77% to 80% with Ca. L. americanus, respectively. Phylogenetic analysis suggested that all Ca. L. asiaticus were clustered into one group, and then clustered in a larger group as Candidatus Liberibacter with Ca. L africanus and Ca. L. americanus.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2011年第2期125-132,共8页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家现代柑橘产业技术体系华东柑橘综合试验站资助项目(农业部农科教发[2007]14号文件)
浙江省农业科学院科技创新能力提升工程资助项目(2009R27Y01D01)
浙江省属公益类科研院所基本科研专项资助项目(2009R10029-3)
关键词
亚洲种黄龙病菌
核糖体蛋白基因
多态性
系统发育分析
Candidatus Liberibacter asiaticus
ribosomal protein genes
polymorphism
phylogenetic analysis
