摘要
以茂名野生动物园斑鼻羚体内分离出的毛首线虫为研究对象,用保守引物PCR扩增其核糖体DNA（rDNA）的内转录间隔区（ITS）和5.8 S序列,并进行克隆、转化、测序和序列分析,对样品进行分子鉴定。结果获得2个毛首线虫样品的ITS及5.8 S rDNA序列,总长为1 316 bp,样品间序列相似性为99.2%。将序列与GenBankTM公布的相关序列进行比较分析,结果显示与羊毛首线虫的ITS1、5.8 S和ITS2序列相似性高,分别为97.3%-97.6%、100%和97.8%-98.0%,表明斑鼻羚体内分离的毛首线虫属于羊毛首线虫。
The aims of this study were to amplify and analyze the sequences of internal transcribed spacers（ITS） of ribosomal DNA（rDNA） of Trichuris samples isolated from a Addax nasomaculatus in Maoming Safari Park,China.The DNA of two samples,namely MM3 and MM4,were extracted and the ITS sequences were amplified by PCR with primers NC5 and NC2.The amplicons were cloned into pGEM-T Easy vector and the inserts were successfully sequenced.The results revealed that the total length of ITS and 5.8 S rDNA of the two Trichuris samples from A.nasomaculatus were 1 316 bp,and with a sequence similarity of 99.2%.The samples were identified as Trichuris ovis based on the sequence similarities of 97.3%-97.6%,100% and 97.8%-98.0% for ITS1,5.8 S and ITS2,respectively,compared with the corresponding ITS sequences of T.ovis published in GenBankTM.It was the first time that the ITS sequence of T.ovis from A.nasomaculatus was reported.The results of the present study provided a foundation for further studies of Trichuris spp.
出处
《动物医学进展》
CSCD
北大核心
2011年第3期47-50,共4页
Progress In Veterinary Medicine
基金
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)
关键词
斑鼻羚
毛首线虫
内转录间隔区
PCR技术
序列分析
Addax nasomaculatus
Trichuris
internal transcribed spacers（ITS）
PCR amplification
sequence analysis
