摘要
目的:观察异甘草酸镁对四氯化碳诱导的肝纤维化大鼠肝脏组织TGF-β1及Smad蛋白表达的影响,以期揭示其抗纤维化的机制。方法:利用腹腔注射四氯化碳(CCl4)建立大鼠肝纤维化模型,然后应用不同剂量的异甘草酸镁和INF-γ处理,于实验第16周末检测大鼠血清透明质酸(HA)、层粘连蛋白(LN)、III型前胶原(PC-III)、IV型胶原(C-IV)的水平,采用RT-PCR法检测TGF-β1,Smad3,Smad7mRNA的表达。结果:与模型组比较,异甘草酸镁各剂量组血清HA,LN,PC-III,C-IV水平显著下降(P<0.05),肝脏TGF-β1、smad3的表达明显降低(P<0.05),smad7则有所上升。结论:异甘草酸镁可以改善肝纤维化大鼠肝组织纤维化程度,其作用机理与抑制TGF-β1、Smad3mRNA的表达,上调Smad7mRNA的表达有密切关系。
Objective：To discuss the probable effects of Magnesium Isoglycyrrhizinate on rats of experimental hepatic fibrosis and its potential molecular mechanisms.Methods：Healthy male SD rats were randomly divided into different groups：normal control,model of hepatic fibrosis,INF-γ-treated and Magnesium Isoglycyrrhizinate-treated groups.Hepatic fibrosis in healthy male SD rats was induced by intraperitoneal injection of Tetrachloride.The serum concentration of Hyaluronic acid（HA）,Laminin（LN）,Procolagen Type III（PCIII） and Collagen Type IV（C-IV） were assayed with Radioimmunoassay.The mRNA levels of TGFβ1,Smad 3,and Smad 7 in rat＇s liver tissue were detected with RT-PCR.Results：Compared with model group,the level of serum HA,LN,PC-III,C-IV in Magnesium Isoglycyrrhizinate groups were significantly decreased（P〈0.05）,the pathological fibrosis scores were also decreased obviously（P〈0.05）,and the expression of TGF-β1,Smad3 mRNA in rat＇s liver tissue were decreased significantly（P〈0.05）,but Smad7 mRNA was increased.Conclusion：The mechanisms of Magnesium Isoglycyrrhizinate on protecting against tetrachloride——induced liver fibrosis in SD rats may be reduced the expression of TGF-β1,Smad3 mRNA,and increase the expression of Smad7 mRNA at the same time.
出处
《现代生物医学进展》
CAS
2011年第2期240-242,共3页
Progress in Modern Biomedicine
