摘要
为探索罗布麻酶法脱胶的最优工艺条件,采用筛选的新型罗布麻脱胶菌种——琼氏不动杆菌(Acinetobacter junii FM208850),在单因素实验基础上,运用Box-Behnken设计的响应面试验方法对酶法脱胶工艺进行了预测。根据回归分析确定了最优工艺:添加质量分数0.2%Na2CO3使沤麻液初始pH值为9.5,酶量8.38 mL(161.3 U/mL),质量体积比1 g∶16 mL,脱胶温度45℃,脱胶时间10 h;此工艺下测得残胶率为7.937%。与回归模型预测残胶率相差不大,模型与实际情况拟合较好。
This manuscript aims to attain the optimum degumming conditions for Apocynum veneturn L by a new strain-Acinetobacter junii FM208850 , the response surface methodology designed by Box-Behnken was applied based on the single factor experiment. According to the regression analysis, the optimal conditions were determined and listed as follows： Na2CO3 was 0. 2 g/100mL H2O to make initial pH 9.5, pectinase amount of 8. 38 mL（161.3 U/mL）, bath ratio of 1 ： 16, temperature of 45 ℃, time of 10 h; The actual residual gum content was 7. 937% under the optimum conditions.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2011年第1期118-123,共6页
Journal of Food Science and Biotechnology
基金
辽宁省纺织材料绿色加工技术创新团队项目(2006.T028)
辽宁省高等学校优秀人才支持计划项目(2006.R13)
关键词
罗布麻
酶法脱胶
响应面
Apocynum venetum L. , enzymatic degumming, response surface methodology
