摘要
目的:探讨参麦注射液预处理对大鼠肝脏缺血再灌注损伤的影响。方法:60只SD大鼠随机分为3组:假手术组、参麦组和缺血再灌注组(NS组)。后两组动物麻醉后经门静脉注入生理盐水或参麦,然后阻断肝蒂造成肝脏缺血;假手术组仅行分离不阻断。恢复供血40 min后采集下腔静脉血测定血清谷丙转氨酶(ALT)、乳酸脱氢酶(LDH),取肝组织ELISA测定超氧化物歧化酶(SOD)与丙二醛(MDA)水平,苏木素-伊红染色观察肝脏病理改变。结果:参麦组大鼠血清ALT、LDH水平及肝组织MDA含量均低于缺血再灌注组(P<0.01),而肝组织SOD含量则高于缺血再灌注组(P<0.01);病理结果显示参麦预处理组肝细胞形态学异常改变较缺血再灌注组明显减轻。结论:参麦预处理对大鼠肝脏缺血再灌注损伤具有保护作用,其机制可能与抑制炎性细胞因子生成及促进抗炎细胞因子表达,以及提高肝组织SOD含量,抑制脂质过氧化有关。
Objective： To investigate the protective effects of shenmai injection （Shenmai） preconditioning against hepatic ischemia - reperfusion injury in rats. Methods ： Sixty male SD rats were allocated into 3 groups ： sham operation group, shenmai precondi- tioning groups, ischcmia - reperfusion group, The latter two groups of rats were injected normal saline or shenmai injection, respectively ; the pedicles of liver were clamped for 45 minutes, and reperfused for 40 min. The portal trial were only separated but not obstructed ~ the serum levels of ALT, LDH, SOD and MDA in liver tissue were examined, and histopathological changes of liver were observed. Results ： Compared with those of ischemia - reperfusion group, the serum levels of ALT, LDH, and the content of MDA in liver tissue of shenmai group were lower （ P 〈0. 01 ）, and the activity of SOD was higher （ P 〈0. 01 ）. The abnormal changes of the morphology of hepatocyte in the shenmai group were improved. Conclusions： Precondition with shenmai injection can increase the activity of SOD in the hepatic tissue, inhibit lipid peroxidation and protect mitochondria, which could lead to reducing the liver injury caused by ischemia - reperfusion.
出处
《中国民族民间医药》
2011年第4期39-40,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
关键词
参麦
缺血再灌注
肝脏
大鼠
shenmai injection, ischemia - reperfusion, liver, rats
