摘要
目的:建立人血浆甲砜霉素检测的高效液相色谱方法。方法:血浆经乙酸乙酯-正己烷萃取,色谱柱:Agilent TC-C_(18);流动相:乙腈-0.1%三氟乙酸-水(20:20:60),流速:0.8 ml·min^(-1);检测波长:225 nm。结果:甲砜霉素浓度在0.10～10.00 mg·L^(-1)范围内线性关系良好(r=0.999 9);高中低三个浓度(0.25,2.50,7.50 mg·L^(-1))的相对回收率分别为(99.20±4.41)%、(98.07±3.94)%和(100.07±1.69)%;日内RSD分别为5.28%、5.19%和1.97%,日间RSD分别为4.38%、3.95%和1.33%。结论:本方法简便、快速、准确可靠,适用于人血浆甲砜霉素浓度的测定及其药代动力学研究。
Objective： To develop an HPLC method for the determination of thiamphenicol in human plasma. Method： The plasma was extracted by ethyl acetate and n-henaxe. The analytical column was packed with Agilent TC-C18. The mobile phase was acetonitrile- 0. 1% trifluoroacetic acid-water （20： 20： 60） and the flow rate was 0. 8 ml .min-1. The UV detection wavelength was 225 nm. Result： Excellent linear relationship was obtained from 0. 10 mg. L-1 to 10. 00 mg. L-1 （ r = 0. 999 9 ）. The relative recovery was （ 99.20 ± 4. 41 ） % , （98. 07 ± 3. 94） % and （ 100. 07± 1.69 ） % at three concentrations, respectively. The intra-day RSD was 5.28%, 5. 19% and 1.97% and inter-day RSD was 4. 38% ,3.95% and 1.33% , respectively. Conclusion： The method is simple, rapid and accurate and can be used to determine the thiarnphenicol concentration in human plasma and study its pharmacokinetics.
出处
《中国药师》
CAS
2011年第2期194-196,共3页
China Pharmacist
基金
温州市科技局计划项目(编号:Y20100137)
