摘要
目的探讨营养状态调节机体生长发育的分子生物学机制。方法利用Northern印迹技术，分析禁食对雄性Wistar大鼠肝胰岛素样生长因子－Ⅰ（IGF－Ⅰ）和胰岛素样生长因子结合蛋白（IGFBP－Ⅰ）基因表达的影响。结果禁食48小时后大鼠体重下降11．8％、血糖下降50.8％；禁食48小时后，肝IGF－ⅠmRNA含量下降32．7％；与此相反，禁食后肝IGBP-1mRNA含量逐渐上升，禁食24小时后IGFBR－ⅠmRNA含量为正常的2．5倍；禁食后肝白蛋白mRNA含量无明显变化。结论禁食抑制大鼠肝IGF－Ⅰ基因表达，促进IGFBF－Ⅰ基因表达。
Objective To investigate the molecular mechanisms of body development and growth regulatedby nutritional status.Methods Effects of fasting on the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ)andIGF binding protein-1 (IGFBP-1)genes were analyzed by northern blotting technique in rats.Results Fasting for48 hours, body weight of the rats decreased by 11.8%,level of blood glucose by 50.8% and content of IGF-ⅠmRNA in the liver by 32.7%(P<0.05).On the contrary, content of IGFBP-1 mRNA in the liver of fasted ratsincreased gradually, with a 2.5 time increase after 24 h fasting (P<0.05). There was no sigificant change inthe level of liver albumin mRNA after fasting. Conclusion Fasting could inhibit the expression of IGF-Ⅰ geneand enhance the expression of IGFBP-1 gene.
出处
《中华预防医学杂志》
CSCD
北大核心
1999年第5期289-291,共3页
Chinese Journal of Preventive Medicine
关键词
禁食
IGF-I
IGFBP-1
基因表达
mRNA
Fasting Insulin-like growth factor Ⅰ Insulin-like growth-factor binding protein 1 Gene expression
